| Summary: | This laboratory experiment was designed to isolate zinc-solubilizing bacteria (ZSB) and evaluate their potential use as a plant growth-promoter. A mineral salts medium (MSM) with the addition of insoluble zinc was used in plate observation of zinc solubilization before the ZSB were characterized further for other biochemical properties. Out of 28 bacteria isolated from rice soil, ten were found to solubilize zinc. Selected bacterial isolate (SR R-10) was the best ZSB, with the significantly highest solubilization efficiency for zinc oxide (558.33%), zinc carbonate (419.33%) and phosphate solubilization (146.67%). In addition, eight ZSB isolates were able to fix nitrogen, and seven tested positive for siderophores production. In terms of phytohormone production, SR R-10 led the production of indole compounds at 6.140 µg/mL, followed by SR R-2 and SR N-3 at 5.865 and 5.167 µg/mL, respectively. A 16S ribosomal ribonucleic acid (rRNA) molecular characterization revealed that SR R-10 as Acinetobacter nosocomialis. This bacterial strain has the potential to be used as a biofertilizer and substitute for chemical fertilizer usage, thus promoting sustainable agricultural practices. © 2023, Khon Kaen University,Research and Technology Transfer Affairs Division. All rights reserved.
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