Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway

Two-third of breast cancer patients expressed estrogen receptors (ER)s and received endocrine treatment with established anti-estrogens such as tamoxifen. But the action and acquired resistance during treatment are largely unknown. In contrary, phytochemicals are more selective and less cytotoxic to...

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Published in:Sains Malaysiana
Main Author: Amin I.M.; Kamaludin R.; Yeap S.K.; Isa M.R.; Mohd Rosdy N.M.M.N.; Siran R.; Kadir S.H.S.A.; Hasani N.A.H.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2015
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942020241&doi=10.17576%2fjsm-2015-4408-09&partnerID=40&md5=95a3dcdbf825c439e17dfebdb74619dc
id 2-s2.0-84942020241
spelling 2-s2.0-84942020241
Amin I.M.; Kamaludin R.; Yeap S.K.; Isa M.R.; Mohd Rosdy N.M.M.N.; Siran R.; Kadir S.H.S.A.; Hasani N.A.H.
Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
2015
Sains Malaysiana
44
8
10.17576/jsm-2015-4408-09
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942020241&doi=10.17576%2fjsm-2015-4408-09&partnerID=40&md5=95a3dcdbf825c439e17dfebdb74619dc
Two-third of breast cancer patients expressed estrogen receptors (ER)s and received endocrine treatment with established anti-estrogens such as tamoxifen. But the action and acquired resistance during treatment are largely unknown. In contrary, phytochemicals are more selective and less cytotoxic to normal cells. Accordingly, we found aloe emodin, an anthraquinone to inhibit the proliferation of ER+-breast cancer cells, MCF-7 with IC50 of 80 μM, but not affecting control breast cells, MCF-10A. Tamoxifen was non-selective to both cells with IC50 of 27 and 38 μM, respectively. Thus, we aimed to investigate the anti-proliferative mechanism of aloe emodin on MCF-7 and its underlying signalling compared to tamoxifen. Cells were treated separately with aloe emodin and tamoxifen at respective IC50 for 72 h. Apoptosis was determined using Annexin V-FITC/PI staining. The expression of insulin-like growth factor-1 receptor (IGF-1R), insulin-like growth factor binding protein (IGFBP)-2 and B-raf gene was investigated using QuantiGene 2.0 Plex assay. Paired-student t-test and ANOVA test were used to compare between untreated and treated cells on the measured parameters. Each treatment was conducted in triplicate and repeated three times. Significance was set at p<0.05. The presences of early and late apoptosis in MCF-7 were seen in both treatments. All target genes were down regulated. The anti-proliferation effect of aloe emodin on MCF-7 is similar with tamoxifen which mediates inhibition of IGF-1R signalling pathway. This suggests aloe emodin as a potential anti-cancer agent to be used in combined anti-estrogen therapy to enhance its efficacy in ER+-breast cancer treatment.
Penerbit Universiti Kebangsaan Malaysia
1266039
English
Article
All Open Access; Gold Open Access
author Amin I.M.; Kamaludin R.; Yeap S.K.; Isa M.R.; Mohd Rosdy N.M.M.N.; Siran R.; Kadir S.H.S.A.; Hasani N.A.H.
spellingShingle Amin I.M.; Kamaludin R.; Yeap S.K.; Isa M.R.; Mohd Rosdy N.M.M.N.; Siran R.; Kadir S.H.S.A.; Hasani N.A.H.
Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
author_facet Amin I.M.; Kamaludin R.; Yeap S.K.; Isa M.R.; Mohd Rosdy N.M.M.N.; Siran R.; Kadir S.H.S.A.; Hasani N.A.H.
author_sort Amin I.M.; Kamaludin R.; Yeap S.K.; Isa M.R.; Mohd Rosdy N.M.M.N.; Siran R.; Kadir S.H.S.A.; Hasani N.A.H.
title Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
title_short Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
title_full Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
title_fullStr Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
title_full_unstemmed Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
title_sort Aloe emodin induces apoptosis in ER+-breast cancer cells; MCF-7 through IGF-1R signalling pathway
publishDate 2015
container_title Sains Malaysiana
container_volume 44
container_issue 8
doi_str_mv 10.17576/jsm-2015-4408-09
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942020241&doi=10.17576%2fjsm-2015-4408-09&partnerID=40&md5=95a3dcdbf825c439e17dfebdb74619dc
description Two-third of breast cancer patients expressed estrogen receptors (ER)s and received endocrine treatment with established anti-estrogens such as tamoxifen. But the action and acquired resistance during treatment are largely unknown. In contrary, phytochemicals are more selective and less cytotoxic to normal cells. Accordingly, we found aloe emodin, an anthraquinone to inhibit the proliferation of ER+-breast cancer cells, MCF-7 with IC50 of 80 μM, but not affecting control breast cells, MCF-10A. Tamoxifen was non-selective to both cells with IC50 of 27 and 38 μM, respectively. Thus, we aimed to investigate the anti-proliferative mechanism of aloe emodin on MCF-7 and its underlying signalling compared to tamoxifen. Cells were treated separately with aloe emodin and tamoxifen at respective IC50 for 72 h. Apoptosis was determined using Annexin V-FITC/PI staining. The expression of insulin-like growth factor-1 receptor (IGF-1R), insulin-like growth factor binding protein (IGFBP)-2 and B-raf gene was investigated using QuantiGene 2.0 Plex assay. Paired-student t-test and ANOVA test were used to compare between untreated and treated cells on the measured parameters. Each treatment was conducted in triplicate and repeated three times. Significance was set at p<0.05. The presences of early and late apoptosis in MCF-7 were seen in both treatments. All target genes were down regulated. The anti-proliferation effect of aloe emodin on MCF-7 is similar with tamoxifen which mediates inhibition of IGF-1R signalling pathway. This suggests aloe emodin as a potential anti-cancer agent to be used in combined anti-estrogen therapy to enhance its efficacy in ER+-breast cancer treatment.
publisher Penerbit Universiti Kebangsaan Malaysia
issn 1266039
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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