| Summary: | Fluorescein and its various derivatives show remarkable biological and bio-analytical applications because of its solubility, high degree of inertness and high fluorescent activity. To broaden the spectrum of its applications, we synthesized novel fluorescein-based hydrazides (FHJ-0.2- FHJ-0.6) using different substituted aryl hydrazine. The newly designed and synthesized products have been monitored by thin layer chromatography and characterized by 1 HNMR, IR and MS data. To find the application of these hydrazides, the biological screening is considered and screened by antimicrobial and hemolytic activity studies. Time-kill assay revealed that none of these hydrazides are appreciable antimicrobial agent but all of these show promising hemolytic results. Results are compared with Triton X-100 (positive control) with 89.54% hemolytic activity. Compared to fluorescein (FHJ-0.1) activity (3.07%), FHJ-0.3(3.54%), FHJ-0.2 (4.37%), FHJ-0.2 (4.37%) and FHJ-0.6 (5.87%), presented better anti- hemolytic activity showing that changing the substituent and position affects the activity remarkably. These fluoresecin based hydrazides may prove to be useful for the treatment of hemolytic disorders.
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