Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan

The high biodiversity of plant species in Malaysian forests provides a suitable environment for the isolation of endophytic Streptomyces. Novel strains of Streptomyces are potential sources for producing bioactive pharmaceutical compounds that can be developed as new drug candidates. This study aims...

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Published in:SAINS MALAYSIANA
Main Authors: Sarmin, Nurul 'Izzah Mohd; Zini, Noraziah mohamad
Format: Article
Language:English
Published: UNIV KEBANGSAAN MALAYSIA, FAC SCIENCE & TECHNOLOGY 2024
Subjects:
Online Access:https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-recordWOS:001367374600018
author Sarmin
Nurul 'Izzah Mohd; Zini
Noraziah mohamad
spellingShingle Sarmin
Nurul 'Izzah Mohd; Zini
Noraziah mohamad
Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
Science & Technology - Other Topics
author_facet Sarmin
Nurul 'Izzah Mohd; Zini
Noraziah mohamad
author_sort Sarmin
spelling Sarmin, Nurul 'Izzah Mohd; Zini, Noraziah mohamad
Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
SAINS MALAYSIANA
English
Article
The high biodiversity of plant species in Malaysian forests provides a suitable environment for the isolation of endophytic Streptomyces. Novel strains of Streptomyces are potential sources for producing bioactive pharmaceutical compounds that can be developed as new drug candidates. This study aims to isolate the endophytic Streptomyces from different medicinal plants in the Bangi Reserve Forest before identification, characterisation, and phylogenetic analysis of the isolates. The endophytic actinomycetes were isolated using surface-sterilization method and further identified through morphological observation. The isolated endophytic Streptomyces were classified based on the colour of aerial mycelium. 16S rRNA gene sequence analysis was done to define the phylogenetic relationships among closely related species and strains belonging to a species. All isolates were then tested for their antimicrobial activities. Three endophytic Streptomyces, SUK 8, SUK 10 and SUK 15 were successfully isolated from different medicinal plants. These three isolates were classified into a grey series group based on the colour of aerial mycelium. The grey series also formed the same phylogenetic clade based on the 16S rRNA sequence data. Phylogenetic analysis of 16S rRNA gene sequences showed all three isolates were classified as Streptomyces eurythermus ATCC 14975T (gene sequence similarity 98.5%). Isolates of SUK 8, SUK 10 and SUK 15 also showed similar patterns of biological activities with inhibition of up to 100% of the same pathogenic organisms. These findings proved a good correlation between the diversity of the antimicrobial phenotype and the 16S rRNA gene. In conclusion, the potential of active metabolites from endophytic Streptomyces can be expected from a good source of taxonomy data.
UNIV KEBANGSAAN MALAYSIA, FAC SCIENCE & TECHNOLOGY
0126-6039

2024
53
11
10.17576/jsm-2024-5311-18
Science & Technology - Other Topics

WOS:001367374600018
https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-recordWOS:001367374600018
title Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
title_short Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
title_full Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
title_fullStr Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
title_full_unstemmed Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
title_sort Pemencilan, Pencirian dan Afiliasi Filogenetik Endofit Streptomyces sp. Bioaktif daripada Pokok yang Mempunyai Nilai Perubatan
container_title SAINS MALAYSIANA
language English
format Article
description The high biodiversity of plant species in Malaysian forests provides a suitable environment for the isolation of endophytic Streptomyces. Novel strains of Streptomyces are potential sources for producing bioactive pharmaceutical compounds that can be developed as new drug candidates. This study aims to isolate the endophytic Streptomyces from different medicinal plants in the Bangi Reserve Forest before identification, characterisation, and phylogenetic analysis of the isolates. The endophytic actinomycetes were isolated using surface-sterilization method and further identified through morphological observation. The isolated endophytic Streptomyces were classified based on the colour of aerial mycelium. 16S rRNA gene sequence analysis was done to define the phylogenetic relationships among closely related species and strains belonging to a species. All isolates were then tested for their antimicrobial activities. Three endophytic Streptomyces, SUK 8, SUK 10 and SUK 15 were successfully isolated from different medicinal plants. These three isolates were classified into a grey series group based on the colour of aerial mycelium. The grey series also formed the same phylogenetic clade based on the 16S rRNA sequence data. Phylogenetic analysis of 16S rRNA gene sequences showed all three isolates were classified as Streptomyces eurythermus ATCC 14975T (gene sequence similarity 98.5%). Isolates of SUK 8, SUK 10 and SUK 15 also showed similar patterns of biological activities with inhibition of up to 100% of the same pathogenic organisms. These findings proved a good correlation between the diversity of the antimicrobial phenotype and the 16S rRNA gene. In conclusion, the potential of active metabolites from endophytic Streptomyces can be expected from a good source of taxonomy data.
publisher UNIV KEBANGSAAN MALAYSIA, FAC SCIENCE & TECHNOLOGY
issn 0126-6039

publishDate 2024
container_volume 53
container_issue 11
doi_str_mv 10.17576/jsm-2024-5311-18
topic Science & Technology - Other Topics
topic_facet Science & Technology - Other Topics
accesstype
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url https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-recordWOS:001367374600018
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