Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies
The aim of this study is to investigate the treatment of burn wounds and bacterial infections, specifically focusing on silver(I) N-heterocyclic carbene (Ag-NHC) loaded into cubosomes. Ag-NHC is an organometallic complex of silver (Ag) and can be considered an active pharmaceutical ingredient for th...
Published in: | INORGANIC CHEMISTRY COMMUNICATIONS |
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Language: | English |
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2024
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Online Access: | https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001360015000001 |
author |
Farooq Umer; Hayat Sofia; Nawaz Maubashera; Nee Tan Wen; Khaw Kooi Yeong; Iqbal Muhammad Adnan; Khalid Syed Haroon; Masood Tanzeela; Ijaz Muhammad Umar; Yaqoob Munazzah |
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spellingShingle |
Farooq Umer; Hayat Sofia; Nawaz Maubashera; Nee Tan Wen; Khaw Kooi Yeong; Iqbal Muhammad Adnan; Khalid Syed Haroon; Masood Tanzeela; Ijaz Muhammad Umar; Yaqoob Munazzah Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies Chemistry |
author_facet |
Farooq Umer; Hayat Sofia; Nawaz Maubashera; Nee Tan Wen; Khaw Kooi Yeong; Iqbal Muhammad Adnan; Khalid Syed Haroon; Masood Tanzeela; Ijaz Muhammad Umar; Yaqoob Munazzah |
author_sort |
Farooq |
spelling |
Farooq, Umer; Hayat, Sofia; Nawaz, Maubashera; Nee, Tan Wen; Khaw, Kooi Yeong; Iqbal, Muhammad Adnan; Khalid, Syed Haroon; Masood, Tanzeela; Ijaz, Muhammad Umar; Yaqoob, Munazzah Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies INORGANIC CHEMISTRY COMMUNICATIONS English Article The aim of this study is to investigate the treatment of burn wounds and bacterial infections, specifically focusing on silver(I) N-heterocyclic carbene (Ag-NHC) loaded into cubosomes. Ag-NHC is an organometallic complex of silver (Ag) and can be considered an active pharmaceutical ingredient for the topical treatment of burns. A novel nanoparticle system, cubosome dispersions, was formulated using high pressure homogenization methods with different concentrations of lipid phase glyceryl monooleate (GMO), surfactants such as poloxamer 407 (F-127), and polyvinyl alcohol (PVA). The optimized formulation (U-6) was characterized by in vitro drug release profile (90.54 +/- 1.17 %), particle size (126.7 +/- 0.98 nm), zeta potential (-21.6 +/- 6.83 mV), entrapment efficiency (89.30 +/- 1.46 %), and morphology as observed by transmission electron microscopy (TEM). The U-6 formulation was incorporated into a hydrogel containing Carbopol 940 to form a cubosomal hydrogel (cubogel). The cubogel was characterized by the in vitro release of Ag-NHC (92 %), entrapment efficiency (>90 %), pH (5.3), viscosity (1.2 cP), spreadability, and TEM. From the in vitro drug release pattern, it was concluded that the U-6 formulation showed slow and sustained drug release over 24 h, and demonstrated superior burn healing compared to marketed products. The in vivo wound healing study revealed that the cubogel demonstrated a superior burn healing rate compared to commercially available products (Quench (R) and Clotrimazole (R)). The in vivo histopathological results showed that the prepared cubogel was effective in treating second-degree burns, with no side effects or skin irritation, compared to commercial products. Additionally, Ag-NHC-loaded cubogel facilitated sustained release for treating burn wounds without any bacterial infections. The current findings provide a strong basis for the initiating phase 0 clinal trials, highlighting significant potential for the further research to benefit the pharmaceutical industry. ELSEVIER 1387-7003 1879-0259 2024 170 10.1016/j.inoche.2024.113504 Chemistry WOS:001360015000001 https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001360015000001 |
title |
Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies |
title_short |
Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies |
title_full |
Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies |
title_fullStr |
Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies |
title_full_unstemmed |
Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies |
title_sort |
Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies |
container_title |
INORGANIC CHEMISTRY COMMUNICATIONS |
language |
English |
format |
Article |
description |
The aim of this study is to investigate the treatment of burn wounds and bacterial infections, specifically focusing on silver(I) N-heterocyclic carbene (Ag-NHC) loaded into cubosomes. Ag-NHC is an organometallic complex of silver (Ag) and can be considered an active pharmaceutical ingredient for the topical treatment of burns. A novel nanoparticle system, cubosome dispersions, was formulated using high pressure homogenization methods with different concentrations of lipid phase glyceryl monooleate (GMO), surfactants such as poloxamer 407 (F-127), and polyvinyl alcohol (PVA). The optimized formulation (U-6) was characterized by in vitro drug release profile (90.54 +/- 1.17 %), particle size (126.7 +/- 0.98 nm), zeta potential (-21.6 +/- 6.83 mV), entrapment efficiency (89.30 +/- 1.46 %), and morphology as observed by transmission electron microscopy (TEM). The U-6 formulation was incorporated into a hydrogel containing Carbopol 940 to form a cubosomal hydrogel (cubogel). The cubogel was characterized by the in vitro release of Ag-NHC (92 %), entrapment efficiency (>90 %), pH (5.3), viscosity (1.2 cP), spreadability, and TEM. From the in vitro drug release pattern, it was concluded that the U-6 formulation showed slow and sustained drug release over 24 h, and demonstrated superior burn healing compared to marketed products. The in vivo wound healing study revealed that the cubogel demonstrated a superior burn healing rate compared to commercially available products (Quench (R) and Clotrimazole (R)). The in vivo histopathological results showed that the prepared cubogel was effective in treating second-degree burns, with no side effects or skin irritation, compared to commercial products. Additionally, Ag-NHC-loaded cubogel facilitated sustained release for treating burn wounds without any bacterial infections. The current findings provide a strong basis for the initiating phase 0 clinal trials, highlighting significant potential for the further research to benefit the pharmaceutical industry. |
publisher |
ELSEVIER |
issn |
1387-7003 1879-0259 |
publishDate |
2024 |
container_volume |
170 |
container_issue |
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doi_str_mv |
10.1016/j.inoche.2024.113504 |
topic |
Chemistry |
topic_facet |
Chemistry |
accesstype |
|
id |
WOS:001360015000001 |
url |
https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001360015000001 |
record_format |
wos |
collection |
Web of Science (WoS) |
_version_ |
1818940501007532032 |