Applications of CRISPR in Parasitology

• Published in: CURRENT PHARMACEUTICAL BIOTECHNOLOGY
• Main Authors: Tee, Phoebe Yon Ern; Chu, Sylvester Yee Chun; Kok, Chloe Chien Yee; Foo, Mun; Tan, Clarence Zhen Jin; Foo, Jhi Biau; Hatta, Fazleen Haslinda Mohd; Tan, Li Kar Stella; Hamzah, Sharina
• Format: Review; Early Access
• Language: English
• Published: BENTHAM SCIENCE PUBL LTD 2024
• Subjects: Biochemistry & Molecular Biology; Pharmacology & Pharmacy
• Online Access: https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001276130600001
• ISSN: 1389-2010; 1873-4316
• DOI: 10.2174/0113892010316710240626042205

Clustered Regions of Interspersed Palindromic Repeat (CRISPR)-based techniques have been utilized in various research areas, including agriculture, biotechnology, and medicine. With the use of a short sequence guide RNA and CRISPR-associated (Cas) protein, this technique allows for robust, site-specific manipulation of the genome, aiding researchers in making important biomedical discoveries and scientific advancements. In this review, we explored the applications of CRISPR/Cas systems in the field of parasitology for the identification and validation of novel functional genes, diagnosis of parasitic infections, reduction of parasite virulence, and the disruption of disease transmission. We also discussed how CRISPR can be used for the development of therapeutics, vaccines, and drug discovery. Furthermore, the challenges and future perspectives of this technology are also highlighted.