ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM

Endophytic fungi of the medicinal herb, Ocimum sanctum are believed to possess antifungal activity against pathogenic fungi. Due to the emergence of pathogenic fungi and antibiotic-resistant strains, the search for alternative antimicrobial agents is a need. The present study aimed to evaluate the a...

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Published in:JURNAL TEKNOLOGI-SCIENCES & ENGINEERING
Main Authors: Jalil, Mohd Taufiq Mat; Yahya, Mohd Fakharul Zaman Raja; Zakaria, Nurul Aili; Ibrahim, Darah
Format: Article
Language:English
Published: PENERBIT UTM PRESS 2024
Subjects:
Online Access:https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001242619000011
author Jalil
Mohd Taufiq Mat; Yahya
Mohd Fakharul Zaman Raja; Zakaria
Nurul Aili; Ibrahim
Darah
spellingShingle Jalil
Mohd Taufiq Mat; Yahya
Mohd Fakharul Zaman Raja; Zakaria
Nurul Aili; Ibrahim
Darah
ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
Engineering
author_facet Jalil
Mohd Taufiq Mat; Yahya
Mohd Fakharul Zaman Raja; Zakaria
Nurul Aili; Ibrahim
Darah
author_sort Jalil
spelling Jalil, Mohd Taufiq Mat; Yahya, Mohd Fakharul Zaman Raja; Zakaria, Nurul Aili; Ibrahim, Darah
ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
JURNAL TEKNOLOGI-SCIENCES & ENGINEERING
English
Article
Endophytic fungi of the medicinal herb, Ocimum sanctum are believed to possess antifungal activity against pathogenic fungi. Due to the emergence of pathogenic fungi and antibiotic-resistant strains, the search for alternative antimicrobial agents is a need. The present study aimed to evaluate the antifungal activity of endophytic fungi isolated from O. sanctum. Plate -to -plate method, disk diffusion assay, and Scanning electron microscopic (SEM) were employed in this study. The finding revealed that fungal isolates Colletotrichum sp. IBRL OS -39, Aspergillus sp. IBRL OS -65, Muscodor sp. IBRL OS -94 and Muscodor sp. IBRL OS -98 was able to produce volatile compounds with antifungal activity against pathogenic fungi. On the disk diffusion assay, Lasiodiplodia sp. IBRL OS -64, and Muscodor sp. IBRL OS -94 displayed good antifungal activity against test fungi with a diameter of inhibition zone between 9.6 +/- 0.6 - 14.3 +/- 0.6 mm and 11.2 +/- 1.2 - 15.7 +/- 0.6 mm, respectively. SEM observations revealed remarkable morphological changes in Candida albicans treated with the dichloromethane extracts of Lasiodiplodia sp. IBRL OS -64 and Muscodor sp. IBRL OS -94 with severe cell damage beyond repair and thus leads to cell death.
PENERBIT UTM PRESS
0127-9696
2180-3722
2024
86
2
10.11113/jurnalteknologi.v86.20508
Engineering
gold
WOS:001242619000011
https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001242619000011
title ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_short ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_full ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_fullStr ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_full_unstemmed ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_sort ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
container_title JURNAL TEKNOLOGI-SCIENCES & ENGINEERING
language English
format Article
description Endophytic fungi of the medicinal herb, Ocimum sanctum are believed to possess antifungal activity against pathogenic fungi. Due to the emergence of pathogenic fungi and antibiotic-resistant strains, the search for alternative antimicrobial agents is a need. The present study aimed to evaluate the antifungal activity of endophytic fungi isolated from O. sanctum. Plate -to -plate method, disk diffusion assay, and Scanning electron microscopic (SEM) were employed in this study. The finding revealed that fungal isolates Colletotrichum sp. IBRL OS -39, Aspergillus sp. IBRL OS -65, Muscodor sp. IBRL OS -94 and Muscodor sp. IBRL OS -98 was able to produce volatile compounds with antifungal activity against pathogenic fungi. On the disk diffusion assay, Lasiodiplodia sp. IBRL OS -64, and Muscodor sp. IBRL OS -94 displayed good antifungal activity against test fungi with a diameter of inhibition zone between 9.6 +/- 0.6 - 14.3 +/- 0.6 mm and 11.2 +/- 1.2 - 15.7 +/- 0.6 mm, respectively. SEM observations revealed remarkable morphological changes in Candida albicans treated with the dichloromethane extracts of Lasiodiplodia sp. IBRL OS -64 and Muscodor sp. IBRL OS -94 with severe cell damage beyond repair and thus leads to cell death.
publisher PENERBIT UTM PRESS
issn 0127-9696
2180-3722
publishDate 2024
container_volume 86
container_issue 2
doi_str_mv 10.11113/jurnalteknologi.v86.20508
topic Engineering
topic_facet Engineering
accesstype gold
id WOS:001242619000011
url https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001242619000011
record_format wos
collection Web of Science (WoS)
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