Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
Background: In a prior study, Lasiodiplodia theobromae (Pat.) Griffiths and Maubl. and Bipolaris bicolor (Mitra) Shoemaker., were found to suppress the growth of Eleusine indica (L.) Gaertn, but limited information exists on their response to environmental factors and herbicides for integrated E. in...
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SOC BRASILEIRA CIENCIA PLANTAS DANINHAS-SBCPD
2024
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Online Access: | https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001202895500001 |
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Fakri Muhammad Aiman; Sapak Zaiton; Hamdani Muhammad Saiful Ahmad; Seng Chuah Tse |
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Fakri Muhammad Aiman; Sapak Zaiton; Hamdani Muhammad Saiful Ahmad; Seng Chuah Tse Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica Plant Sciences |
author_facet |
Fakri Muhammad Aiman; Sapak Zaiton; Hamdani Muhammad Saiful Ahmad; Seng Chuah Tse |
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Fakri |
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Fakri, Muhammad Aiman; Sapak, Zaiton; Hamdani, Muhammad Saiful Ahmad; Seng, Chuah Tse Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica ADVANCES IN WEED SCIENCE English Article Background: In a prior study, Lasiodiplodia theobromae (Pat.) Griffiths and Maubl. and Bipolaris bicolor (Mitra) Shoemaker., were found to suppress the growth of Eleusine indica (L.) Gaertn, but limited information exists on their response to environmental factors and herbicides for integrated E. indica control. Objective: This study aimed to determine the tolerance levels of L. theobromae and B. bicolor to pH, temperature, photoperiod, relative humidity, and herbicides. Methods: The mycelia and conidia of L. theobromae and B. bicolor were exposed to a range of environmental conditions and herbicides in a controlled setting, including different levels of temperature (25, 30, 35 0C), pH (4, 6, 8, 10), photoperiod (24 hours of darkness; alternating 12 hours of blue light followed by 12 hours of darkness; and alternating cycles of 6 hours of blue light and 6 hours of darkness), and relative humidity (75, 85, 95%). Herbicides such as topramezone, diuron, oxyfluorfen, and imazethapyr were applied at their recommended rates. Conidial germination was assessed by counting after an 18-hour incubation using a haemocytometer, while the diameter of mycelium growth was measured after 3 days of incubation, except for the herbicide effects evaluation, which were extended over 7 days. Results: Laboratory assays demonstrated that L. theobromae exhibited higher conidial germination of 85-95% and superior mycelial growth under varied pH, temperature, and photoperiod conditions compared to B. bicolor. Lasiodioplodia theobromae's mycelia remained unaffected by herbicides at full labelled rates, but they inhibited the conidial germination of the fungus. For B. bicolor , the conidia were not affected by imazethapyr and topramezone, but its mycelial growth was reduced by imazethapyr, oxyfluorfen, and diuron. Conclusions: These results indicate that the mycelia of L. theobromae presents a more favorable option for tank mixing with test herbicides, offering potential for the formulation of an integrated control strategy against E. indica. SOC BRASILEIRA CIENCIA PLANTAS DANINHAS-SBCPD 2675-9462 2024 42 10.51694/AdvWeedSci/2024;42:00005 Plant Sciences gold WOS:001202895500001 https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001202895500001 |
title |
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica |
title_short |
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica |
title_full |
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica |
title_fullStr |
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica |
title_full_unstemmed |
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica |
title_sort |
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica |
container_title |
ADVANCES IN WEED SCIENCE |
language |
English |
format |
Article |
description |
Background: In a prior study, Lasiodiplodia theobromae (Pat.) Griffiths and Maubl. and Bipolaris bicolor (Mitra) Shoemaker., were found to suppress the growth of Eleusine indica (L.) Gaertn, but limited information exists on their response to environmental factors and herbicides for integrated E. indica control. Objective: This study aimed to determine the tolerance levels of L. theobromae and B. bicolor to pH, temperature, photoperiod, relative humidity, and herbicides. Methods: The mycelia and conidia of L. theobromae and B. bicolor were exposed to a range of environmental conditions and herbicides in a controlled setting, including different levels of temperature (25, 30, 35 0C), pH (4, 6, 8, 10), photoperiod (24 hours of darkness; alternating 12 hours of blue light followed by 12 hours of darkness; and alternating cycles of 6 hours of blue light and 6 hours of darkness), and relative humidity (75, 85, 95%). Herbicides such as topramezone, diuron, oxyfluorfen, and imazethapyr were applied at their recommended rates. Conidial germination was assessed by counting after an 18-hour incubation using a haemocytometer, while the diameter of mycelium growth was measured after 3 days of incubation, except for the herbicide effects evaluation, which were extended over 7 days. Results: Laboratory assays demonstrated that L. theobromae exhibited higher conidial germination of 85-95% and superior mycelial growth under varied pH, temperature, and photoperiod conditions compared to B. bicolor. Lasiodioplodia theobromae's mycelia remained unaffected by herbicides at full labelled rates, but they inhibited the conidial germination of the fungus. For B. bicolor , the conidia were not affected by imazethapyr and topramezone, but its mycelial growth was reduced by imazethapyr, oxyfluorfen, and diuron. Conclusions: These results indicate that the mycelia of L. theobromae presents a more favorable option for tank mixing with test herbicides, offering potential for the formulation of an integrated control strategy against E. indica. |
publisher |
SOC BRASILEIRA CIENCIA PLANTAS DANINHAS-SBCPD |
issn |
2675-9462 |
publishDate |
2024 |
container_volume |
42 |
container_issue |
|
doi_str_mv |
10.51694/AdvWeedSci/2024;42:00005 |
topic |
Plant Sciences |
topic_facet |
Plant Sciences |
accesstype |
gold |
id |
WOS:001202895500001 |
url |
https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001202895500001 |
record_format |
wos |
collection |
Web of Science (WoS) |
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1809678907271544832 |