Styryllactones in the leaves of Goniothalamus lanceolatus Miq., molecular networking and their anti-dengue activity In vitro and In silico

• Published in: PHYTOCHEMISTRY LETTERS
• Main Authors: Abdullah, Nor Nadirah; Afzan, Adlin; Jelas, Nur Hana; Abd Razak, Mohd Ridzuan Mohd; Rasol, Nurulfazlina Edayah; Abu Bakar, Syahrul Imran; Bihud, Nur Vicky; Wai, Lam Kok; Zainol, Murizal; Ahmad, Fasihuddin Badruddin; Cordell, Geoffrey A.; Ismail, Nor Hadiani
• Format: Article
• Language: English
• Published: ELSEVIER 2024
• Subjects: Plant Sciences; Pharmacology & Pharmacy
• Online Access: https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001185300100001

Due to dengue fever's swift global expansion and the lack of effective antiviral remedies, it is crucial to discover and develop new antiviral drugs. The study aimed to assess the antiviral potential of the extract and fractions from the leaves of Goniothalamus lanceolatus Miq. (Annonaceae). New Guinea C strain DENV-2, at a multiplicity of infection of 0.4, was used to infect Vero cells. The assessment of antiviral effectiveness was conducted through the plaque reduction assay. Deep metabolome analysis of the active fraction identified nine styryllactones based on reference standards and eighteen styryllactones were further annotated through a molecular database search. One new 2H-tetrahydropyran derivative, 3-epi-goniothalesdiol A (19), together with seven known styryllactones (8, 11, 14, 16, 17, 18, and 22) were isolated from the leaves of G. lanceolatus. The active bis-styryllactone goniolanceolatin A (22) with the highest selectivity index (SI) underwent further evaluation using quantitative reverse transcription qRT-PCR to determine the viral RNA level. The qRT-PCR data showed that the IC50 value for the active compound was 5.07 mu g/mL, and its corresponding SI value was 5.30. Following comprehensive docking studies, the bis-styryllactone derivative 22 showed potential binding interactions with crucial amino acids of the Envelope (E) of DENV proteins comparable to those of ribavirin.