Protection of Fargesin against Hydrogen Peroxide-induced Cell Death in RAW 264.7 Cells

• Published in: INTERNATIONAL JOURNAL OF PHARMACEUTICAL INVESTIGATION
• Main Authors: Roof, Amira Nabila Mat; James, Richard Johari; Ramli, Salfarina; Sritularak, Boonchoo; Rojsitthisak, Pornchai; Halim, Hasseri
• Format: Article
• Language: English
• Published: INPHARM ASSOC, PHCOG NET 2024
• Subjects: Pharmacology & Pharmacy
• Online Access: https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001165156000028
• ISSN: 2230-973X; 2230-9713
• DOI: 10.5530/ijpi.14.1.8

Background: The imbalance level of Reactive Oxygen Species (ROS) in the body can lead to oxidative stress in cells, resulting in the progression of chronic diseases. Fargesin, a bioactive lignan found in Flos magnoliae, an herb widely used in Chinese herbal medicines, has been identified as a potential compound to mitigate this imbalance. Materials and Methods: Hydrogen peroxide (H2O2), one of the major ROS, is commonly employed as an experimental model to induce oxidative stress. This study aimed to investigate the protective effect of fargesin against cell death induced by hydrogen peroxide in RAW 264.7 macrophage cells. RAW 264.7 macrophage cells were pre-treated with various concentrations of fargesin before being exposed to toxic dose (1 mM or 2 mM) of hydrogen peroxide. The viability of the cells was assessed using the MTT assay, and the morphological changes were described. Results and Discussion: The MTT assay demonstrated that non-toxic doses of fargesin (1.56 mu M, 3.13 mu M, 6.25 mu M, 12.5 mu M, and 25 mu M) significantly reduced H2O2-induced cytotoxicity in the treated cells compared to the control group. Conclusion: This research is expected to provide a basis for future studies in the development of a new antioxidant agent for the treatment and prevention of diseases associated with oxidative stress.