Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs

Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclu...

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Published in:SCIENTIFIC REPORTS
Main Authors: Dinter, Carl; Gumprecht, Andreas; Menze, Matthias Alexander; Azizan, Amizon; Niehoff, Paul-Joachim; Hansen, Sven; Buechs, Jochen
Format: Article
Language:English
Published: NATURE PORTFOLIO 2024
Subjects:
Online Access:https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001162143900044
author Dinter
Carl; Gumprecht
Andreas; Menze
Matthias Alexander; Azizan
Amizon; Niehoff
Paul-Joachim; Hansen
Sven; Buechs
Jochen
spellingShingle Dinter
Carl; Gumprecht
Andreas; Menze
Matthias Alexander; Azizan
Amizon; Niehoff
Paul-Joachim; Hansen
Sven; Buechs
Jochen
Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
Science & Technology - Other Topics
author_facet Dinter
Carl; Gumprecht
Andreas; Menze
Matthias Alexander; Azizan
Amizon; Niehoff
Paul-Joachim; Hansen
Sven; Buechs
Jochen
author_sort Dinter
spelling Dinter, Carl; Gumprecht, Andreas; Menze, Matthias Alexander; Azizan, Amizon; Niehoff, Paul-Joachim; Hansen, Sven; Buechs, Jochen
Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
SCIENTIFIC REPORTS
English
Article
Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclusively concentrated on fluids at waterlike viscosity. The importance of CFD model validation cannot be overstated. While techniques to elucidate the internal flow field are necessary for model validation in STRs, the liquid distribution, caused by the orbital shaking motion of shake flasks, can be exploited for model validation. An OpenFOAM CFD model for shake flasks has been established. Calculated liquid distributions were compared to suitable, previously published experimental data. Across a broad range of shaking conditions, at waterlike and moderate viscosity (16.7 mPa center dot s), the CFD model's liquid distributions align excellently with the experimental data, in terms of overall shape and position of the liquid relative to the direction of the centrifugal force. Additionally, the CFD model was used to calculate the volumetric power input, based on the energy dissipation. Depending on the shaking conditions, the computed volumetric power inputs range from 0.1 to 7 kW/m3 and differed on average by 0.01 kW/m3 from measured literature data.
NATURE PORTFOLIO
2045-2322

2024
14
1
10.1038/s41598-024-53980-7
Science & Technology - Other Topics
gold
WOS:001162143900044
https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001162143900044
title Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_short Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_full Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_fullStr Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_full_unstemmed Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_sort Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
container_title SCIENTIFIC REPORTS
language English
format Article
description Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclusively concentrated on fluids at waterlike viscosity. The importance of CFD model validation cannot be overstated. While techniques to elucidate the internal flow field are necessary for model validation in STRs, the liquid distribution, caused by the orbital shaking motion of shake flasks, can be exploited for model validation. An OpenFOAM CFD model for shake flasks has been established. Calculated liquid distributions were compared to suitable, previously published experimental data. Across a broad range of shaking conditions, at waterlike and moderate viscosity (16.7 mPa center dot s), the CFD model's liquid distributions align excellently with the experimental data, in terms of overall shape and position of the liquid relative to the direction of the centrifugal force. Additionally, the CFD model was used to calculate the volumetric power input, based on the energy dissipation. Depending on the shaking conditions, the computed volumetric power inputs range from 0.1 to 7 kW/m3 and differed on average by 0.01 kW/m3 from measured literature data.
publisher NATURE PORTFOLIO
issn 2045-2322

publishDate 2024
container_volume 14
container_issue 1
doi_str_mv 10.1038/s41598-024-53980-7
topic Science & Technology - Other Topics
topic_facet Science & Technology - Other Topics
accesstype gold
id WOS:001162143900044
url https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001162143900044
record_format wos
collection Web of Science (WoS)
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