Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent inflammation of the sinonasal mucosa. CRSwNP treatments are associated with inconsistent efficacy and recurrence of symptoms. Dynorphin 1-17 (DYN 1-17) and its fragments have been shown to modulate the immune response in various infla...

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Published in:ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Main Authors: Ballouze, Rama; Ismail, Mohd Nazri; Abu Kassim, Nur Sofiah; Salhimi, Salizawati Muhamad; Mohamad, Irfan; Abd Mutalib, Nor Shahida; Hassim, Ahmad Anuar; Rahiman, Siti Sarah Fazalul
Format: Article; Early Access
Language:English
Published: SPRINGER HEIDELBERG 2023
Subjects:
Online Access:https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001111974100001
author Ballouze
Rama; Ismail
Mohd Nazri; Abu Kassim
Nur Sofiah; Salhimi
Salizawati Muhamad; Mohamad
Irfan; Abd Mutalib
Nor Shahida; Hassim
Ahmad Anuar; Rahiman
Siti Sarah Fazalul
spellingShingle Ballouze
Rama; Ismail
Mohd Nazri; Abu Kassim
Nur Sofiah; Salhimi
Salizawati Muhamad; Mohamad
Irfan; Abd Mutalib
Nor Shahida; Hassim
Ahmad Anuar; Rahiman
Siti Sarah Fazalul
Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
Biochemistry & Molecular Biology; Chemistry
author_facet Ballouze
Rama; Ismail
Mohd Nazri; Abu Kassim
Nur Sofiah; Salhimi
Salizawati Muhamad; Mohamad
Irfan; Abd Mutalib
Nor Shahida; Hassim
Ahmad Anuar; Rahiman
Siti Sarah Fazalul
author_sort Ballouze
spelling Ballouze, Rama; Ismail, Mohd Nazri; Abu Kassim, Nur Sofiah; Salhimi, Salizawati Muhamad; Mohamad, Irfan; Abd Mutalib, Nor Shahida; Hassim, Ahmad Anuar; Rahiman, Siti Sarah Fazalul
Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
English
Article; Early Access
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent inflammation of the sinonasal mucosa. CRSwNP treatments are associated with inconsistent efficacy and recurrence of symptoms. Dynorphin 1-17 (DYN 1-17) and its fragments have been shown to modulate the immune response in various inflammatory conditions. This study aimed to investigate the effect of different pH and degrees of inflammation on DYN 1-17 metabolism in human CRSwNP tissues. DYN 1-17 was incubated with grade 3 and grade 4 inflamed tissues of CRSwNP patients at pH 5.5 and pH 7.4 over a range of incubation periods. The resulting fragments were identified using an ultra-performance liquid chromatography (UPLC) system coupled to quadrupole-time of flight (QTOF) mass spectrometry based on their accurate mass. The rate of DYN 1-17 fragmentation was slower at pH 5.5 in comparison to pH 7.4. The extent and rate of metabolism of DYN 1-17 were much lower in grade 3 inflamed tissue (31-32 fragments) than in grade 4 (34-41 fragments). N-Terminal fragments (DYN 1-15, 1-11, 1-10, and 1-6) were metabolized slower at pH 5.5 as compared to pH 7.4. DYN 1-12, 1-8, 2-10, 4-10, 5-10, and 8-14 were only observed under the inflammatory pH while DYN 5-17 and 6-17 were only identified upon incubation with grade 4 CRSwNP tissues. DYN 1-17 metabolism was significantly affected by the pH level and the severity of the inflammation of CRSwNP tissues, indicating the potential roles of DYN 1-17 and its fragments in modulating the inflammatory response and their avenue as therapeutics in future studies.
SPRINGER HEIDELBERG
1618-2642
1618-2650
2023


10.1007/s00216-023-05061-3
Biochemistry & Molecular Biology; Chemistry

WOS:001111974100001
https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001111974100001
title Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
title_short Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
title_full Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
title_fullStr Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
title_full_unstemmed Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
title_sort Detection of dynorphin 1-17 biotransformation fragments in human nasal polyps by UPLC-QTOF-MS
container_title ANALYTICAL AND BIOANALYTICAL CHEMISTRY
language English
format Article; Early Access
description Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent inflammation of the sinonasal mucosa. CRSwNP treatments are associated with inconsistent efficacy and recurrence of symptoms. Dynorphin 1-17 (DYN 1-17) and its fragments have been shown to modulate the immune response in various inflammatory conditions. This study aimed to investigate the effect of different pH and degrees of inflammation on DYN 1-17 metabolism in human CRSwNP tissues. DYN 1-17 was incubated with grade 3 and grade 4 inflamed tissues of CRSwNP patients at pH 5.5 and pH 7.4 over a range of incubation periods. The resulting fragments were identified using an ultra-performance liquid chromatography (UPLC) system coupled to quadrupole-time of flight (QTOF) mass spectrometry based on their accurate mass. The rate of DYN 1-17 fragmentation was slower at pH 5.5 in comparison to pH 7.4. The extent and rate of metabolism of DYN 1-17 were much lower in grade 3 inflamed tissue (31-32 fragments) than in grade 4 (34-41 fragments). N-Terminal fragments (DYN 1-15, 1-11, 1-10, and 1-6) were metabolized slower at pH 5.5 as compared to pH 7.4. DYN 1-12, 1-8, 2-10, 4-10, 5-10, and 8-14 were only observed under the inflammatory pH while DYN 5-17 and 6-17 were only identified upon incubation with grade 4 CRSwNP tissues. DYN 1-17 metabolism was significantly affected by the pH level and the severity of the inflammation of CRSwNP tissues, indicating the potential roles of DYN 1-17 and its fragments in modulating the inflammatory response and their avenue as therapeutics in future studies.
publisher SPRINGER HEIDELBERG
issn 1618-2642
1618-2650
publishDate 2023
container_volume
container_issue
doi_str_mv 10.1007/s00216-023-05061-3
topic Biochemistry & Molecular Biology; Chemistry
topic_facet Biochemistry & Molecular Biology; Chemistry
accesstype
id WOS:001111974100001
url https://www-webofscience-com.uitm.idm.oclc.org/wos/woscc/full-record/WOS:001111974100001
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collection Web of Science (WoS)
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