Gelam honey promotes ex vivo corneal fibroblasts wound healing

• 出版年: Cytotechnology
• 第一著者: 2-s2.0-85074598785
• フォーマット: 論文
• 言語: English
• 出版事項: Springer 2019
• オンライン･アクセス: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85074598785&doi=10.1007%2fs10616-019-00349-8&partnerID=40&md5=b479404d5d9eb1e7ae54d83e76306d5f

This study evaluated the effects of Gelam honey (GH) on ex vivo corneal fibroblast ulcer model via wound healing assay, gene expression and immunocytochemistry. Corneal fibroblasts from New Zealand white rabbits were culture expanded. The corneal fibroblast wound healing capacity was observed by creating a circular wound onto confluent monolayer cells cultured in basal medium (BM), BM with GH, serum-enriched basal medium (BMS) and BMS with GH respectively. Wound healing assay and phenotypic characterization of the corneal fibroblast were performed at different stages of wound closure. Expression of aldehyde dehydrogenase (ALDH), vimentin, α-smooth muscle actin (α-SMA), lumican, collagen I and matrix metalloproteinase 12 (MMP 12) were measured at day 1, day 3 and complete wound closure day. Corneal fibroblast cultured in BMS with GH demonstrated the fastest wound closure, at day 5 post wounding. The gene expressions of ALDH and vimentin were higher than control groups while α-SMA expression was lower, in GH enriched media. The expressions of lumican, collagen I and MMP 12 were also higher in cells cultured in GH enriched media compared to the control groups. GH was shown to promote in vitro corneal fibroblast wound healing and may be a potential natural adjunct in the treatment of corneal wound. © 2019, Springer Nature B.V.