Genetic assemblage of Sarcocystis spp. in Malaysian snakes
Background: Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored. Methods. In this study, fecal specimens of several snake species in Ma...
出版年: | Parasites and Vectors |
---|---|
第一著者: | |
フォーマット: | 論文 |
言語: | English |
出版事項: |
2013
|
オンライン・アクセス: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883509869&doi=10.1186%2f1756-3305-6-257&partnerID=40&md5=ecffdba62e504d19e28b2acc40a10041 |
id |
Lau Y.L.; Chang P.Y.; Subramaniam V.; Ng Y.H.; Mahmud R.; Ahmad A.F.; Fong M.Y. |
---|---|
spelling |
Lau Y.L.; Chang P.Y.; Subramaniam V.; Ng Y.H.; Mahmud R.; Ahmad A.F.; Fong M.Y. 2-s2.0-84883509869 Genetic assemblage of Sarcocystis spp. in Malaysian snakes 2013 Parasites and Vectors 6 1 10.1186/1756-3305-6-257 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883509869&doi=10.1186%2f1756-3305-6-257&partnerID=40&md5=ecffdba62e504d19e28b2acc40a10041 Background: Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored. Methods. In this study, fecal specimens of several snake species in Malaysia were examined for the presence of Sarcocystis by PCR of 18S rDNA sequence. Microscopy examination of the fecal specimens for sporocysts was not carried as it was difficult to determine the species of the infecting Sarcocystis. Results: Of the 28 snake fecal specimens, 7 were positive by PCR. BLASTn and phylogenetic analyses of the amplified 18S rDNA sequences revealed the snakes were infected with either S. nesbitti, S. singaporensis, S. zuoi or undefined Sarcocystis species. Conclusion: This study is the first to report Sarcocystis infection in a cobra, and S. nesbitti in a reticulated python. © 2013 Lau et al.; licensee BioMed Central Ltd. 17563305 English Article All Open Access; Gold Open Access; Green Open Access |
author |
2-s2.0-84883509869 |
spellingShingle |
2-s2.0-84883509869 Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
author_facet |
2-s2.0-84883509869 |
author_sort |
2-s2.0-84883509869 |
title |
Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
title_short |
Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
title_full |
Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
title_fullStr |
Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
title_full_unstemmed |
Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
title_sort |
Genetic assemblage of Sarcocystis spp. in Malaysian snakes |
publishDate |
2013 |
container_title |
Parasites and Vectors |
container_volume |
6 |
container_issue |
1 |
doi_str_mv |
10.1186/1756-3305-6-257 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883509869&doi=10.1186%2f1756-3305-6-257&partnerID=40&md5=ecffdba62e504d19e28b2acc40a10041 |
description |
Background: Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored. Methods. In this study, fecal specimens of several snake species in Malaysia were examined for the presence of Sarcocystis by PCR of 18S rDNA sequence. Microscopy examination of the fecal specimens for sporocysts was not carried as it was difficult to determine the species of the infecting Sarcocystis. Results: Of the 28 snake fecal specimens, 7 were positive by PCR. BLASTn and phylogenetic analyses of the amplified 18S rDNA sequences revealed the snakes were infected with either S. nesbitti, S. singaporensis, S. zuoi or undefined Sarcocystis species. Conclusion: This study is the first to report Sarcocystis infection in a cobra, and S. nesbitti in a reticulated python. © 2013 Lau et al.; licensee BioMed Central Ltd. |
publisher |
|
issn |
17563305 |
language |
English |
format |
Article |
accesstype |
All Open Access; Gold Open Access; Green Open Access |
record_format |
scopus |
collection |
Scopus |
_version_ |
1828987883359305728 |