Genetic assemblage of Sarcocystis spp. in Malaysian snakes

Background: Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored. Methods. In this study, fecal specimens of several snake species in Ma...

وصف كامل

التفاصيل البيبلوغرافية
الحاوية / القاعدة:Parasites and Vectors
المؤلف الرئيسي: 2-s2.0-84883509869
التنسيق: مقال
اللغة:English
منشور في: 2013
الوصول للمادة أونلاين:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883509869&doi=10.1186%2f1756-3305-6-257&partnerID=40&md5=ecffdba62e504d19e28b2acc40a10041
الوصف
الملخص:Background: Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored. Methods. In this study, fecal specimens of several snake species in Malaysia were examined for the presence of Sarcocystis by PCR of 18S rDNA sequence. Microscopy examination of the fecal specimens for sporocysts was not carried as it was difficult to determine the species of the infecting Sarcocystis. Results: Of the 28 snake fecal specimens, 7 were positive by PCR. BLASTn and phylogenetic analyses of the amplified 18S rDNA sequences revealed the snakes were infected with either S. nesbitti, S. singaporensis, S. zuoi or undefined Sarcocystis species. Conclusion: This study is the first to report Sarcocystis infection in a cobra, and S. nesbitti in a reticulated python. © 2013 Lau et al.; licensee BioMed Central Ltd.
تدمد:17563305
DOI:10.1186/1756-3305-6-257