| Summary: | Asiaticoside is one of the biomarker components in Centella asiatica that possesses extraordinary medicinal properties. It can be used as a source of natural ingredients in the pharmaceutical and cosmeceutical industries. In this work, asiaticoside was extracted using ultrasound and betaine-based natural deep eutectic solvent (NADES). The kinetics of asiaticoside extraction was studied using Peleg’s model. The influence of the extraction temperature, ultrasound power, and liquid-to-solid ratio on the model constants was investigated. The experimental data had good agreement with the predicted data, with R2 > 0.90 and small RMSRE values (between 0.01 and 0.031), indicating the high reliability of the model in describing the extraction process of asiaticoside. The C. asiatica extract had a DPPH IC50 value of 8.63 ± 0.88 mg/mL and a total phenolic content of 160.56 ± 0.44 mg GAE/g. In addition, asiaticoside was recovered from the betaine-based NADES using macroporous adsorption resin. The effects of the amount of resin, extract dilution, ethanol concentration, and ethanol volume on the static adsorption/desorption performance were investigated. The highest recovery percentage of asiaticoside (71.36 ± 0.51%) was obtained under the following conditions: 2 g of resin, 80% (v/v) diluted extract, 90% aqueous ethanol, and 50 mL of desorption solvent. This green extraction technique offers high extraction efficiency and high-quality extracts. Meanwhile, HPD 100 macroporous resin successfully recovered asiaticoside from the betaine-based NADES. © King Fahd University of Petroleum & Minerals 2025.
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