| Summary: | Introduction. The consumption of fructose in excessive quantities has been implicated in the onset of obesity and a spectrum of metabolic dysfunctions. Physical exercise is posited as a potent intervention to ameliorate obesity-induced metabolic anomalies, ostensibly through the elevation of adiponectin concentrations. However, the underlying molecular mechanisms of this effect remain inadequately understood. Objective. This study aims to demonstrate the impact of exercise intensity on increasing adiponectin levels in high-fructose-induced mice, highlighting the underlying molecular mechanisms. Methods. The experiment was carried out on 36 male mice (Mus musculus), aged ±8 weeks, with body weight ± 20 – 25 grams, in healthy condition and without defects. Mice were randomly divided into four groups. Control group without training (CN; n = 9); the low-intensity swimming training group with a 3% load of the mice’s body weight (LI; n = 9); the moderate-intensity swimming training group with a 6% load of the mice’s body weight (MI; n = 9); the heavy intensity swimming training group with a 9% load of the mice’s body weight (HI; n = 9). The frequency of swimming training was carried out 3 times/week for 8 weeks, and the duration of swimming training was calculated as 80% of the maximum swimming time every session. All groups were orally (oral ad libitum) given 30% fructose solution for 8 weeks. Adiponectin levels were quantified via ELISA. Statistical interrogation employed one-way ANOVA and Tukey’s HSD post hoc test, with a significance threshold set at 5%. Results. The results indicated a statistically significant divergence in adiponectin levels (p ≤ 0.001). Tukey’s HSD post hoc test analysis revealed substantial disparities between CN and LI (p = 0.196), CN and MI (p = 0.0001), CN and HI (p = 0.001), LI and MI (p = 0.001), LI and HI (p = 0.001), and MI and HI (p = 0.001). Conclusion. This study found that moderate-intensity swimming training was more optimal in increasing adiponectin levels in fructose-induced mice compared to highintensity, low-intensity, and control groups. Additionally, this research identified specific molecular pathways activated by moderate-intensity training, providing new insights for therapeutic interventions in tackling obesity-related metabolic dysfunctions. © 2025. María Cano University Foundation.
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