Role of CRISPR-Cas System as a New Approach in Fighting the Antimicrobial Resistance of Bacterial and Viral Pathogens

• Published in: Infectious Diseases and Immunity
• Main Author: Selim H.M.R.M.; Gomaa F.A.M.; Alshahrani M.Y.; Aboshanab K.M.
• Format: Review
• Language: English
• Published: Wolters Kluwer Health 2024
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85212832521&doi=10.1097%2fID9.0000000000000149&partnerID=40&md5=fd6e38d8bb72cad711a0f92bd1548178

The clustered regularly interspaced short palindromic repeat (CRISPR)-Caspase (Cas) system acts as a natural defense of bacteria against invasion by mobile genetic elements, such as plasmids, transposons, and bacteriophages. The review discusses the different classes and types of CRISPR-Cas systems in terms of principles of their action, limitations, and future perspectives. Also, the role of the CRISPR-Cas system as a new arsenal in fighting multidrug-resistant pathogens and clinically relevant pathogenic viruses and up-To-date clinical trials have been discussed and highlighted. Moreover, the utilization mode, regulation, and the link of CRISPR-Cas to quorum sensing for targeting cell chromosome or antimicrobial-resistant gene(s) of some clinically relevant pathogens, including Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa have been discussed. The information provided in this review can act as a platform for researchers for the future use of the CRISPR-Cas system as a smart antimicrobial agent for combating infections caused by life-Threatening pathogens, particularly those with limited therapeutic options. © 2024 Wolters Kluwer Health. All rights reserved.