In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare
Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in si...
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Malaysian Society of Applied Biology
2024
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2-s2.0-85206373169 Nematiasgarabad P.; Hashim N.A.N.; Yahya M.F.Z.R. In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare 2024 Malaysian Applied Biology 53 3 10.55230/mabjournal.v53i3.2942 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206373169&doi=10.55230%2fmabjournal.v53i3.2942&partnerID=40&md5=3423905794b050bf1ebf91573650e301 Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in silico studies have provided various biological insights into F. columnare, including its interaction with MHC class I alleles and the epitopic region within outer membrane proteins. However, the protein-protein interaction networks underlying the growth, defense, and pathogenesis of F. columnare remain largely unknown. This study was conducted to identify the protein-protein interaction (PPI) networks and hub proteins of F. columnare that can be used as drug or vaccine targets. A total of 500 protein sequences were retrieved from UniprotKB in FASTA format and analyzed using VaxiJen, PSORTb, STRING, Cytoscape, and BLASTp programs. The results demonstrated that 60% of F. columnare proteins were predicted as antigenic proteins, most of which were associated with catalytic activity and metabolic processes, identified as cytoplasmic proteins. Ten hub proteins with the highest number of functional interactions were identified, which were also antigenic and non-host homologous. In conclusion, F. columnare hub proteins represent potential therapeutic targets in drug and vaccine development against columnaris infection. © 2024 Malaysian Society of Applied Biology. Malaysian Society of Applied Biology 01268643 English Article All Open Access; Hybrid Gold Open Access |
author |
Nematiasgarabad P.; Hashim N.A.N.; Yahya M.F.Z.R. |
spellingShingle |
Nematiasgarabad P.; Hashim N.A.N.; Yahya M.F.Z.R. In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
author_facet |
Nematiasgarabad P.; Hashim N.A.N.; Yahya M.F.Z.R. |
author_sort |
Nematiasgarabad P.; Hashim N.A.N.; Yahya M.F.Z.R. |
title |
In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
title_short |
In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
title_full |
In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
title_fullStr |
In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
title_full_unstemmed |
In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
title_sort |
In Silico Elucidation of Protein-Protein Interaction Network in Fish Pathogen Flavobacterium columnare |
publishDate |
2024 |
container_title |
Malaysian Applied Biology |
container_volume |
53 |
container_issue |
3 |
doi_str_mv |
10.55230/mabjournal.v53i3.2942 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206373169&doi=10.55230%2fmabjournal.v53i3.2942&partnerID=40&md5=3423905794b050bf1ebf91573650e301 |
description |
Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in silico studies have provided various biological insights into F. columnare, including its interaction with MHC class I alleles and the epitopic region within outer membrane proteins. However, the protein-protein interaction networks underlying the growth, defense, and pathogenesis of F. columnare remain largely unknown. This study was conducted to identify the protein-protein interaction (PPI) networks and hub proteins of F. columnare that can be used as drug or vaccine targets. A total of 500 protein sequences were retrieved from UniprotKB in FASTA format and analyzed using VaxiJen, PSORTb, STRING, Cytoscape, and BLASTp programs. The results demonstrated that 60% of F. columnare proteins were predicted as antigenic proteins, most of which were associated with catalytic activity and metabolic processes, identified as cytoplasmic proteins. Ten hub proteins with the highest number of functional interactions were identified, which were also antigenic and non-host homologous. In conclusion, F. columnare hub proteins represent potential therapeutic targets in drug and vaccine development against columnaris infection. © 2024 Malaysian Society of Applied Biology. |
publisher |
Malaysian Society of Applied Biology |
issn |
01268643 |
language |
English |
format |
Article |
accesstype |
All Open Access; Hybrid Gold Open Access |
record_format |
scopus |
collection |
Scopus |
_version_ |
1814778497935081472 |