| Summary: | Different extraction methods to screen the phytochemicals of Moringa oleifera (M. oleifera) leaves extracts have been conducted. This study aims to employ both maceration and ultrasonic-assisted techniques, followed by the phytochem-ical screening of the extracts by using thin-layer chromatography (TLC) and High-Performance Liquid Chromatography (HPLC). Compared to sonication, the macer-ation produced a larger yield of extracted material. The TLC analysis showed that a mobile phase consisting of toluene, butanol, and methanol (90:5:5) was the best solvent system for separating most of the constituents in the extracts. The TLC and HPLC profiles demonstrate that neither extraction procedure exhibits notable differences. The HPLC profile of gradient elution with the addition of 0.3% formic acid resulted in greater peak separation and more peak detection, as compared to the isocratic system. Quercetin was not identified as a significant constituent in this research sample, due to the relatively low absorbance. The findings can provide infor-mation on the pharmacological function of M. oleifera leaves, which are potentially valuable source in pharmaceutical and traditional medicines. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2023.
|
|---|