ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING

ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING

Bacillus cereus is a spore-forming food-borne pathogen that can cause food poisoning. The bacteria can produce toxins that lead to emetic and diarrheal in toxication. This study was designed to investigate the occurrence of B. cereus in different types of cereals and vegetables purchased from local...

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Published in:Malaysian Journal of Biochemistry and Molecular Biology
Main Author: Abidin N.Z.; Wathiqah Fadzillah N.F.; Suhaimi M.S.
Format: Article
Language:English
Published: Malaysian Society for Biochemistry and Molecular Biology 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204061055&partnerID=40&md5=9ab5ba037e02d1c8047e1ce65d23a63e
id 2-s2.0-85204061055
spelling 2-s2.0-85204061055
Abidin N.Z.; Wathiqah Fadzillah N.F.; Suhaimi M.S.
ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
2023
Malaysian Journal of Biochemistry and Molecular Biology
2023


https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204061055&partnerID=40&md5=9ab5ba037e02d1c8047e1ce65d23a63e
Bacillus cereus is a spore-forming food-borne pathogen that can cause food poisoning. The bacteria can produce toxins that lead to emetic and diarrheal in toxication. This study was designed to investigate the occurrence of B. cereus in different types of cereals and vegetables purchased from local markets in Negeri Sembilan using selective medium agar, biochemical approaches and bacterial species confirmation using 16S rDNA amplicon sequencing. Phylogenetic tree was then created using MEGA X software to identify relationship between different bacterial species. From this study, the prevalence of B. cereus in cereal and vegetables were recorded at 38.5% and 9.1%, respectively. These suggest that the presence of B. cereus-containing cereals and vegetables in diets may represent the risk in the case of inadequate heat treatment. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
Malaysian Society for Biochemistry and Molecular Biology
15112616
English
Article
author Abidin N.Z.; Wathiqah Fadzillah N.F.; Suhaimi M.S.
spellingShingle Abidin N.Z.; Wathiqah Fadzillah N.F.; Suhaimi M.S.
ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
author_facet Abidin N.Z.; Wathiqah Fadzillah N.F.; Suhaimi M.S.
author_sort Abidin N.Z.; Wathiqah Fadzillah N.F.; Suhaimi M.S.
title ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
title_short ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
title_full ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
title_fullStr ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
title_full_unstemmed ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
title_sort ISOLATION AND IDENTIFICATION OF Bacillus cereus s.l. FROM VEGETABLES AND CEREALS FROM LOCAL MARKETS IN NEGERI SEMBILAN USING 16S rDNA AMPLICON SEQUENCING
publishDate 2023
container_title Malaysian Journal of Biochemistry and Molecular Biology
container_volume 2023
container_issue
doi_str_mv
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204061055&partnerID=40&md5=9ab5ba037e02d1c8047e1ce65d23a63e
description Bacillus cereus is a spore-forming food-borne pathogen that can cause food poisoning. The bacteria can produce toxins that lead to emetic and diarrheal in toxication. This study was designed to investigate the occurrence of B. cereus in different types of cereals and vegetables purchased from local markets in Negeri Sembilan using selective medium agar, biochemical approaches and bacterial species confirmation using 16S rDNA amplicon sequencing. Phylogenetic tree was then created using MEGA X software to identify relationship between different bacterial species. From this study, the prevalence of B. cereus in cereal and vegetables were recorded at 38.5% and 9.1%, respectively. These suggest that the presence of B. cereus-containing cereals and vegetables in diets may represent the risk in the case of inadequate heat treatment. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
publisher Malaysian Society for Biochemistry and Molecular Biology
issn 15112616
language English
format Article
accesstype
record_format scopus
collection Scopus
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