| Summary: | Bacillus cereus is a spore-forming food-borne pathogen that can cause food poisoning. The bacteria can produce toxins that lead to emetic and diarrheal in toxication. This study was designed to investigate the occurrence of B. cereus in different types of cereals and vegetables purchased from local markets in Negeri Sembilan using selective medium agar, biochemical approaches and bacterial species confirmation using 16S rDNA amplicon sequencing. Phylogenetic tree was then created using MEGA X software to identify relationship between different bacterial species. From this study, the prevalence of B. cereus in cereal and vegetables were recorded at 38.5% and 9.1%, respectively. These suggest that the presence of B. cereus-containing cereals and vegetables in diets may represent the risk in the case of inadequate heat treatment. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
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