Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia

A Trypanosoma screening was conducted on 130 pools comprising 1,241 ticks, collected from 674 selected farm ruminants in Peninsular Malaysia. Of these, nine pools were tested positive for Trypanosoma. Subsequent BLAST searches revealed that the 18S rRNA gene sequences were closely related to Trypano...

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Published in:Acta Tropica
Main Author: Kazim A.R.; Low V.L.; Houssaini J.; Tappe D.; Heo C.C.
Format: Article
Language:English
Published: Elsevier B.V. 2024
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203657869&doi=10.1016%2fj.actatropica.2024.107383&partnerID=40&md5=cc4dc58409920ce86a074eb7ac9fffe9
id 2-s2.0-85203657869
spelling 2-s2.0-85203657869
Kazim A.R.; Low V.L.; Houssaini J.; Tappe D.; Heo C.C.
Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
2024
Acta Tropica
259

10.1016/j.actatropica.2024.107383
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203657869&doi=10.1016%2fj.actatropica.2024.107383&partnerID=40&md5=cc4dc58409920ce86a074eb7ac9fffe9
A Trypanosoma screening was conducted on 130 pools comprising 1,241 ticks, collected from 674 selected farm ruminants in Peninsular Malaysia. Of these, nine pools were tested positive for Trypanosoma. Subsequent BLAST searches revealed that the 18S rRNA gene sequences were closely related to Trypanosoma rhipicephalis isolate Chaco CB, with percentage similarities ranging from 95.56 % to 99.84 %. Phylogenetic analysis showed that three of the nine sequences formed a clade with Trypanosoma rhipicephalis. The remaining six Trypanosoma sequences formed a distinct clade, separate from T. rhipicephalis and other Trypanosoma species, with genetic distances of 4.34 % and 4.33–4.58 %, respectively. This study marks the first report of tick-associated Trypanosoma in Malaysia and underscores significant research gaps regarding trypanosome interactions with tick hosts in the region. © 2024 Elsevier B.V.
Elsevier B.V.
0001706X
English
Article

author Kazim A.R.; Low V.L.; Houssaini J.; Tappe D.; Heo C.C.
spellingShingle Kazim A.R.; Low V.L.; Houssaini J.; Tappe D.; Heo C.C.
Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
author_facet Kazim A.R.; Low V.L.; Houssaini J.; Tappe D.; Heo C.C.
author_sort Kazim A.R.; Low V.L.; Houssaini J.; Tappe D.; Heo C.C.
title Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
title_short Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
title_full Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
title_fullStr Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
title_full_unstemmed Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
title_sort Tick-associated Trypanosoma species (Apicomplexa: Kinetoplastida) from cattle ticks (Acari: Ixodidae) in Peninsular Malaysia
publishDate 2024
container_title Acta Tropica
container_volume 259
container_issue
doi_str_mv 10.1016/j.actatropica.2024.107383
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203657869&doi=10.1016%2fj.actatropica.2024.107383&partnerID=40&md5=cc4dc58409920ce86a074eb7ac9fffe9
description A Trypanosoma screening was conducted on 130 pools comprising 1,241 ticks, collected from 674 selected farm ruminants in Peninsular Malaysia. Of these, nine pools were tested positive for Trypanosoma. Subsequent BLAST searches revealed that the 18S rRNA gene sequences were closely related to Trypanosoma rhipicephalis isolate Chaco CB, with percentage similarities ranging from 95.56 % to 99.84 %. Phylogenetic analysis showed that three of the nine sequences formed a clade with Trypanosoma rhipicephalis. The remaining six Trypanosoma sequences formed a distinct clade, separate from T. rhipicephalis and other Trypanosoma species, with genetic distances of 4.34 % and 4.33–4.58 %, respectively. This study marks the first report of tick-associated Trypanosoma in Malaysia and underscores significant research gaps regarding trypanosome interactions with tick hosts in the region. © 2024 Elsevier B.V.
publisher Elsevier B.V.
issn 0001706X
language English
format Article
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