MULTIPLE REACTION MONITORING (MRM) OF PORCINE-SPECIFIC PEPTIDE MARKERS FROM LACTATE DEHYDROGENASE AND SERUM ALBUMIN IN PROCESSED MEAT FOR HALAL AUTHENTICATION; [Pemantauan Tindak Balas Pelbagai (MRM) Penanda Peptida Khusus bagi Khinzir dari Lactate Dehydrogenase dan Serum Albumin dalam Daging yang Diproses untuk Pengesahan Halal]

• Published in: Malaysian Journal of Analytical Sciences
• Main Author: Abidin S.A.S.Z.; Sazili A.Q.; Faradalila W.N.; Amin I.; Karsani S.A.
• Format: Article
• Language: English
• Published: Malaysian Society of Analytical Sciences 2024
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203294202&partnerID=40&md5=9f5ed5848cd6c98e4fdde5d6d5b7fa21

Multiple Reaction Monitoring (MRM) of four peptides is presented to detect the presence of pork in meat products using a triple quadrupole system (LC-QQQ-MS). Commercial samples were digested using the tryptic digestion method. MRM acquisition was optimized using Skyline software. Then, the presence of peptides was tested and verified on processed meat products, each with a different type of processing. The result reveals that peptides EVTEFAK (m/z = 450.2873), LVVITAGAR (m/z = 412.2134), FVIER (m/z = 388.7369), and TVLGNFAAFVQK (m/z = 647.8613) were consistently detected in the processed meat and displayed porcine-specific properties. Peptide LVVITAGAR, the most intense peptide, was eluted at 7 min while FVIEIR, as the second highest peak, was eluted at 9.5 min. On the other hand, peptide EVTEFAK was eluted at 4.5 min, and TVLGNFAAFVQK, a peptide with the lowest intensity, appeared between 12.5 to 13 min. Specifically, one peptide is derived from lactate dehydrogenase, and three from serum albumin protein. It demonstrated that porcine-specific peptides could be simultaneously targeted by the MRM method with the help of Skyline software that helps optimize the analytes' specific precursor ion, product ion, and fragmentation behaviour under collision energy. The present result reveals that MRM will enable rapid halal detection of contaminants occurring during the manufacturing or supply chain and ensure food integrity is preserved along the food supply chain. © 2024, Malaysian Society of Analytical Sciences. All rights reserved.