IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus]
Staphylococcus aureus is a Gram-positive pathogen inhabiting soft tissues like the epidermis and nasal cavity. Currently, there is limited knowledge of the protein-protein interaction (PPI) networks in S. aureus biofilm. The present study aimed to characterize S. aureus proteins and their interactio...
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Malaysian Society of Analytical Sciences
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2-s2.0-85203268835 Zulkiply N.; Yunus N.M.; Idris H.; Noor W.S.A.W.M.; Karsani S.A.; Yahya M.F.Z.R. IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] 2024 Malaysian Journal of Analytical Sciences 28 4 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203268835&partnerID=40&md5=804d9f6cc8008e9c7fd851b7845edd42 Staphylococcus aureus is a Gram-positive pathogen inhabiting soft tissues like the epidermis and nasal cavity. Currently, there is limited knowledge of the protein-protein interaction (PPI) networks in S. aureus biofilm. The present study aimed to characterize S. aureus proteins and their interaction networks using an in silico approach and to identify the proteins expressed in S. aureus biofilm using tandem mass spectrometry. Initially, a preliminary characterization of the PPI networks in S. aureus was conducted using the STRING 12.0 database. Subsequently, S. aureus biofilm was developed in a 6-well microplate and harvested at 6 h, 12 h, 18 h, and 24 h. The expression of proteins in S. aureus biofilm was determined using a combination of one-dimensional SDS-PAGE and HPLC-ESI-MS/MS. The in silico results demonstrated that 147 biological processes, 46 molecular functions, 17 cellular components, and 15 biological pathways were significantly enriched (p <0.05) in the PPI networks of S. aureus. S. aureus biofilm proteins identified from the SDS-PAGE gel bands, such as L-lactate dehydrogenase (quinone), chaperone protein DnaK, and serine hydroxymethyltransferase, corroborated the findings obtained from the preliminary in silico work. In conclusion, the formation of biofilm by S. aureus may involve complex PPI networks. © 2024, Malaysian Society of Analytical Sciences. All rights reserved. Malaysian Society of Analytical Sciences 13942506 English Article |
author |
Zulkiply N.; Yunus N.M.; Idris H.; Noor W.S.A.W.M.; Karsani S.A.; Yahya M.F.Z.R. |
spellingShingle |
Zulkiply N.; Yunus N.M.; Idris H.; Noor W.S.A.W.M.; Karsani S.A.; Yahya M.F.Z.R. IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
author_facet |
Zulkiply N.; Yunus N.M.; Idris H.; Noor W.S.A.W.M.; Karsani S.A.; Yahya M.F.Z.R. |
author_sort |
Zulkiply N.; Yunus N.M.; Idris H.; Noor W.S.A.W.M.; Karsani S.A.; Yahya M.F.Z.R. |
title |
IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
title_short |
IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
title_full |
IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
title_fullStr |
IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
title_full_unstemmed |
IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
title_sort |
IN SILICO AND MS/MS-BASED APPROACHES TO INVESTIGATE PROTEIN-PROTEIN INTERACTION NETWORKS IN Staphylococcus aureus BIOFILM; [Pendekatan Berasaskan In Silico dan MS/MS Untuk Mengkaji Jaringan Interaksi Protein-Protein dalam Biofilem Staphylococcus aureus] |
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2024 |
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Malaysian Journal of Analytical Sciences |
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28 |
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4 |
doi_str_mv |
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url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203268835&partnerID=40&md5=804d9f6cc8008e9c7fd851b7845edd42 |
description |
Staphylococcus aureus is a Gram-positive pathogen inhabiting soft tissues like the epidermis and nasal cavity. Currently, there is limited knowledge of the protein-protein interaction (PPI) networks in S. aureus biofilm. The present study aimed to characterize S. aureus proteins and their interaction networks using an in silico approach and to identify the proteins expressed in S. aureus biofilm using tandem mass spectrometry. Initially, a preliminary characterization of the PPI networks in S. aureus was conducted using the STRING 12.0 database. Subsequently, S. aureus biofilm was developed in a 6-well microplate and harvested at 6 h, 12 h, 18 h, and 24 h. The expression of proteins in S. aureus biofilm was determined using a combination of one-dimensional SDS-PAGE and HPLC-ESI-MS/MS. The in silico results demonstrated that 147 biological processes, 46 molecular functions, 17 cellular components, and 15 biological pathways were significantly enriched (p <0.05) in the PPI networks of S. aureus. S. aureus biofilm proteins identified from the SDS-PAGE gel bands, such as L-lactate dehydrogenase (quinone), chaperone protein DnaK, and serine hydroxymethyltransferase, corroborated the findings obtained from the preliminary in silico work. In conclusion, the formation of biofilm by S. aureus may involve complex PPI networks. © 2024, Malaysian Society of Analytical Sciences. All rights reserved. |
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Malaysian Society of Analytical Sciences |
issn |
13942506 |
language |
English |
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scopus |
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Scopus |
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1818940557728153600 |