RAPID IDENTIFICATION AND CHARACTERIZATION OF THE Lactobacillus STRAINS BY USING 16S-23S rRNA GENE INTERGENIC SPACER REGION AND recA GENES

• Published in: Malaysian Journal of Biochemistry and Molecular Biology
• Main Author: Ida Muryany M.Y.; Chin Y.K.; Ina-Salwany M.Y.
• Format: Article
• Language: English
• Published: Malaysian Society for Biochemistry and Molecular Biology 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200883257&partnerID=40&md5=b9580668cdf8621ac7ecdf4a19860cca

Lactic acid bacteria are known as potential probiotics that contribute beneficial effects for human beings and farmed animals. Identification of potential Lactic acid bacteria isolates was important for potential probiotic characterization. The isolates were under microscopic determination by Gram staining and electron screening microscopy to determine the morphology of the isolate, followed by rapid identification by using PCR amplification with ITS and recA genes to identify bacteria species of the isolates. Isolates L8 and L20 showed rod-shaped cells in the cluster while isolate S1 showed coccobacillus-shaped cells after the microscopic observation. L8 and L20 both isolates were identified as Lactobacillus plantarum while isolate S1 was identified as Lactobacillus pentosus after PCR amplification with ITS and recA genes. Our present experiment shows that ITS and recA genes can be used with PCR amplification to identify probiotic, Lactobacillus spp accurately. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.