Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma

Humanised xenograft models and cancer cell lines are widely used for preclinical drug evaluation, biological studies, and targeted therapy strategies in cancer research. A humanised mouse model is a laboratory mouse that has been genetically modified to contain specific human genes, cells, or tissue...

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Published in:Immunotherapy Advances
Main Author: Khamarudin F.; Muhamad M.; Ibahim M.J.; I’zzah Wan Mohamad Zain W.N.; Aziz M.A.; Adib Ridzuan N.R.; Ab-Rahim S.
Format: Article
Language:English
Published: Oxford University Press 2024
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85195209308&doi=10.1093%2fimmadv%2fltae002&partnerID=40&md5=29bcd96738de1f2bf3f35b2eaef90770
id 2-s2.0-85195209308
spelling 2-s2.0-85195209308
Khamarudin F.; Muhamad M.; Ibahim M.J.; I’zzah Wan Mohamad Zain W.N.; Aziz M.A.; Adib Ridzuan N.R.; Ab-Rahim S.
Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
2024
Immunotherapy Advances
4
1
10.1093/immadv/ltae002
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85195209308&doi=10.1093%2fimmadv%2fltae002&partnerID=40&md5=29bcd96738de1f2bf3f35b2eaef90770
Humanised xenograft models and cancer cell lines are widely used for preclinical drug evaluation, biological studies, and targeted therapy strategies in cancer research. A humanised mouse model is a laboratory mouse that has been genetically modified to contain specific human genes, cells, or tissues. By introducing human-specific elements into rodents, researchers can create a more accurate representation of human physiological and pathological processes. Lacking an appropriate animal model for osteosarcoma (OS), hindered understanding of underlying mechanisms in OS metastasis progression. Markedly, metastasis influences the prognosis and treatment of osteosarcoma. Gaining insight into the mechanisms and occurrences of metastasis could potentially facilitate oncologists in improving therapies. Hence, it is important to develop a lung metastatic OS model to study the basic biology of its progression. This study has established a tumour-bearing mouse model using HOS-143B cell line which was injected into male NOD.SCID gamma (NSG) mice at two locations; intramuscularly (hind leg) and subcutaneously (back) respectively. The primary and metastatic tumour size was monitored by palpating the area of tumour induced and quantified using digital calliper. H&E staining was performed by pathologist to confirm metastasis. Our results showed that mice injected with 1 million cancer cells were unable to produce tumours. Meanwhile, mice injected with three million cancer cells showed tumour development and lung metastasis after 25 days of cancer cell inoculation. In conclusion, this study has successfully established a lung metastatic OS mouse model that could be useful for biological studies of OS. These findings imply that this model is essential for safety and efficacy before clinical trials, accelerate the translation from basic research to therapeutic applications. © The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology.
Oxford University Press
27324303
English
Article
All Open Access; Gold Open Access
author Khamarudin F.; Muhamad M.; Ibahim M.J.; I’zzah Wan Mohamad Zain W.N.; Aziz M.A.; Adib Ridzuan N.R.; Ab-Rahim S.
spellingShingle Khamarudin F.; Muhamad M.; Ibahim M.J.; I’zzah Wan Mohamad Zain W.N.; Aziz M.A.; Adib Ridzuan N.R.; Ab-Rahim S.
Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
author_facet Khamarudin F.; Muhamad M.; Ibahim M.J.; I’zzah Wan Mohamad Zain W.N.; Aziz M.A.; Adib Ridzuan N.R.; Ab-Rahim S.
author_sort Khamarudin F.; Muhamad M.; Ibahim M.J.; I’zzah Wan Mohamad Zain W.N.; Aziz M.A.; Adib Ridzuan N.R.; Ab-Rahim S.
title Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
title_short Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
title_full Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
title_fullStr Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
title_full_unstemmed Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
title_sort Establishment of humanised xenograft models as in vivo study for lung metastasis of osteosarcoma
publishDate 2024
container_title Immunotherapy Advances
container_volume 4
container_issue 1
doi_str_mv 10.1093/immadv/ltae002
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85195209308&doi=10.1093%2fimmadv%2fltae002&partnerID=40&md5=29bcd96738de1f2bf3f35b2eaef90770
description Humanised xenograft models and cancer cell lines are widely used for preclinical drug evaluation, biological studies, and targeted therapy strategies in cancer research. A humanised mouse model is a laboratory mouse that has been genetically modified to contain specific human genes, cells, or tissues. By introducing human-specific elements into rodents, researchers can create a more accurate representation of human physiological and pathological processes. Lacking an appropriate animal model for osteosarcoma (OS), hindered understanding of underlying mechanisms in OS metastasis progression. Markedly, metastasis influences the prognosis and treatment of osteosarcoma. Gaining insight into the mechanisms and occurrences of metastasis could potentially facilitate oncologists in improving therapies. Hence, it is important to develop a lung metastatic OS model to study the basic biology of its progression. This study has established a tumour-bearing mouse model using HOS-143B cell line which was injected into male NOD.SCID gamma (NSG) mice at two locations; intramuscularly (hind leg) and subcutaneously (back) respectively. The primary and metastatic tumour size was monitored by palpating the area of tumour induced and quantified using digital calliper. H&E staining was performed by pathologist to confirm metastasis. Our results showed that mice injected with 1 million cancer cells were unable to produce tumours. Meanwhile, mice injected with three million cancer cells showed tumour development and lung metastasis after 25 days of cancer cell inoculation. In conclusion, this study has successfully established a lung metastatic OS mouse model that could be useful for biological studies of OS. These findings imply that this model is essential for safety and efficacy before clinical trials, accelerate the translation from basic research to therapeutic applications. © The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology.
publisher Oxford University Press
issn 27324303
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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