| Summary: | Biosurfactants still continue to be an interest for several industry sectors, considering their eco-friendly natures and wide range of applications. However, the production of this natural product is still low, so investigating the optimal conditions for biosurfactant production is still challenging. This research focuses on the production of biosurfactants using indigenous bacteria, Staphylococcus Arlette MPP-1. Optimisation was performed in order to find out the best production, which included glucose concentration, pH, and nitrogen source type. Moreover, the potency of biosurfactants in emulsification was examined and compared to distilled water. Thus, the biosurfactant activity was validated based on the results of the emulsification tests. Based on the results, the highest emulsification activity was obtained from a medium containing 6% glucose (w/v), 0.2% NaNO3(w/v), and pH 6. The emulsification index (EI24) showed more than 70% after 96 hours of incubation using a temperature of 37 oC and an aeration speed of 100 rpm. Preliminary structural analysis through FTIR showed some of the following absorption bands include 3,303 cm-1 O-H stretching, the stretching bands of the amide and ester C=O groups at approximately 1,669 and 1,090 cm-1 as well as N-H and C-N-H vibrations at around 1,636 and 559 cm-1, respectively. © 2024 Author(s).
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