| Summary: | Catechins are known as potent antioxidants with numerous health benefits, including antimicrobial, antiviral, anti-inflammatory, anti-allergenic, and anti-cancer effects. However, there are some challenges to delivering catechins due to their low bioavailability, poor gastrointestinal absorption, and low stability. To overcome the limitations of catechin delivery, nanoemulsion technology with droplet sizes ranging from 10 to 1000 nm was used. In this study, water-in-oil (W/O) nanoemulsion was effectively prepared by employing palm oil (PO) as the oil phase, Span 80 as a lipophilic emulsifier, and Tween 80 as a hydrophilic emulsifier with high-energy approaches (high shear homogenizer). The optimum processing conditions for preparing water-in-palm oil nanoemulsion are as follows: the ratio of the oil phase to the water phase is 60:40, and the total concentrations of emulsifier mixtures are 11 w/w% with a hydrophilic-lipophilic balance (HLB) value of 7.1 using 15,500 rpm for 5 minutes of the high shear homogenizer. The best nanoemulsion showed an average of 185.6 nm particle size and a zeta potential of-38.4 mV. The optimized nanoemulsion containing catechin with a pH value of 6.82, showed a low conductivity value of this nanoemulsion (0.001mS/cm) which means that the continuous phase is oil. The nanoemulsion was stable at centrifugation of 4000rpm for 30 minutes and alongside 28 days of storage tests at 10˚C. Hence, this study successfully developed a stable water-in-palm oil nanoemulsion containing catechins (WPOC-NEs). © 2024, Malaysian Society of Analytical Sciences. All rights reserved.
|
|---|