Gut Microbiota Profiles in Myopes and Nonmyopes
PURPOSE. To identify compositional differences in the gut microbiome of nonmyopes (NM) and myopes using 16S ribosomal RNA sequencing and to investigate whether the microbiome may contribute to the onset or progression of the condition. METHODS. Faecal samples were collected from 52 adult participant...
Published in: | Investigative Ophthalmology and Visual Science |
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Association for Research in Vision and Ophthalmology Inc.
2024
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2-s2.0-85192033751 Omar W.E.W.; Singh G.; McBain A.J.; Cruickshank F.; Radhakrishnan H. Gut Microbiota Profiles in Myopes and Nonmyopes 2024 Investigative Ophthalmology and Visual Science 65 5 10.1167/iovs.65.5.2 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85192033751&doi=10.1167%2fiovs.65.5.2&partnerID=40&md5=3eadb905caf6108cb3852946bd777317 PURPOSE. To identify compositional differences in the gut microbiome of nonmyopes (NM) and myopes using 16S ribosomal RNA sequencing and to investigate whether the microbiome may contribute to the onset or progression of the condition. METHODS. Faecal samples were collected from 52 adult participants, of whom 23 were NM, 8 were progressive myopes (PM), and 21 were stable myopes (SM). The composition of the gut microbiota in each group was analysed using 16S ribosomal RNA gene sequencing. RESULTS. There were no significant differences in alpha and beta diversity between the three groups (NM, PM, and SM). However, the distributions of Bifidobacterium, Bacteroides, Megamonas, Faecalibacterium, Coprococcus, Dorea, Roseburia, and Blautia were significantly higher in the myopes (SM and PM combined) when compared with emmetropes. The myopes exhibited significantly greater abundance of bacteria that are linked to the regulation of dopaminergic signalling, such as Clostridium, Ruminococcus, Bifidobacterium, and Bacteroides. Individuals with stable myopia were found to have a significantly higher proportion of Prevotella copri than those with progressive myopia. Bifidobacterium adolescentis, a gamma-aminobutyric acid (GABA)–producing bacterium, was significantly higher in all myopes than in NM and, in the comparison between SM and PM, it is significantly higher in SM. B. uniformis and B. fragilis, both GABA-producing Bacteroides, were present in relatively high abundance in all myopes and in SM compared with PM, respectively. CONCLUSIONS. The presence of bacteria related to dopamine effect and GABA-producing bacteria in the gut microbiome of myopes may suggest a role of these microorganisms in the onset and progression of myopia. © 2024 Association for Research in Vision and Ophthalmology Inc.. All rights reserved. Association for Research in Vision and Ophthalmology Inc. 1460404 English Article All Open Access; Gold Open Access |
author |
Omar W.E.W.; Singh G.; McBain A.J.; Cruickshank F.; Radhakrishnan H. |
spellingShingle |
Omar W.E.W.; Singh G.; McBain A.J.; Cruickshank F.; Radhakrishnan H. Gut Microbiota Profiles in Myopes and Nonmyopes |
author_facet |
Omar W.E.W.; Singh G.; McBain A.J.; Cruickshank F.; Radhakrishnan H. |
author_sort |
Omar W.E.W.; Singh G.; McBain A.J.; Cruickshank F.; Radhakrishnan H. |
title |
Gut Microbiota Profiles in Myopes and Nonmyopes |
title_short |
Gut Microbiota Profiles in Myopes and Nonmyopes |
title_full |
Gut Microbiota Profiles in Myopes and Nonmyopes |
title_fullStr |
Gut Microbiota Profiles in Myopes and Nonmyopes |
title_full_unstemmed |
Gut Microbiota Profiles in Myopes and Nonmyopes |
title_sort |
Gut Microbiota Profiles in Myopes and Nonmyopes |
publishDate |
2024 |
container_title |
Investigative Ophthalmology and Visual Science |
container_volume |
65 |
container_issue |
5 |
doi_str_mv |
10.1167/iovs.65.5.2 |
url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85192033751&doi=10.1167%2fiovs.65.5.2&partnerID=40&md5=3eadb905caf6108cb3852946bd777317 |
description |
PURPOSE. To identify compositional differences in the gut microbiome of nonmyopes (NM) and myopes using 16S ribosomal RNA sequencing and to investigate whether the microbiome may contribute to the onset or progression of the condition. METHODS. Faecal samples were collected from 52 adult participants, of whom 23 were NM, 8 were progressive myopes (PM), and 21 were stable myopes (SM). The composition of the gut microbiota in each group was analysed using 16S ribosomal RNA gene sequencing. RESULTS. There were no significant differences in alpha and beta diversity between the three groups (NM, PM, and SM). However, the distributions of Bifidobacterium, Bacteroides, Megamonas, Faecalibacterium, Coprococcus, Dorea, Roseburia, and Blautia were significantly higher in the myopes (SM and PM combined) when compared with emmetropes. The myopes exhibited significantly greater abundance of bacteria that are linked to the regulation of dopaminergic signalling, such as Clostridium, Ruminococcus, Bifidobacterium, and Bacteroides. Individuals with stable myopia were found to have a significantly higher proportion of Prevotella copri than those with progressive myopia. Bifidobacterium adolescentis, a gamma-aminobutyric acid (GABA)–producing bacterium, was significantly higher in all myopes than in NM and, in the comparison between SM and PM, it is significantly higher in SM. B. uniformis and B. fragilis, both GABA-producing Bacteroides, were present in relatively high abundance in all myopes and in SM compared with PM, respectively. CONCLUSIONS. The presence of bacteria related to dopamine effect and GABA-producing bacteria in the gut microbiome of myopes may suggest a role of these microorganisms in the onset and progression of myopia. © 2024 Association for Research in Vision and Ophthalmology Inc.. All rights reserved. |
publisher |
Association for Research in Vision and Ophthalmology Inc. |
issn |
1460404 |
language |
English |
format |
Article |
accesstype |
All Open Access; Gold Open Access |
record_format |
scopus |
collection |
Scopus |
_version_ |
1809677881879560192 |