ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM

Endophytic fungi of the medicinal herb, Ocimum sanctum are believed to possess antifungal activity against pathogenic fungi. Due to the emergence of pathogenic fungi and antibiotic-resistant strains, the search for alternative antimicrobial agents is a need. The present study aimed to evaluate the a...

Full description

Bibliographic Details
Published in:Jurnal Teknologi
Main Author: Jalil M.T.M.; Yahya M.F.Z.R.; Zakaria N.A.; Ibrahim D.
Format: Article
Language:English
Published: Penerbit UTM Press 2024
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185653830&doi=10.11113%2fjurnalteknologi.v86.20508&partnerID=40&md5=108a2ed2d5b9629088398271ec87a050
id 2-s2.0-85185653830
spelling 2-s2.0-85185653830
Jalil M.T.M.; Yahya M.F.Z.R.; Zakaria N.A.; Ibrahim D.
ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
2024
Jurnal Teknologi
86
2
10.11113/jurnalteknologi.v86.20508
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185653830&doi=10.11113%2fjurnalteknologi.v86.20508&partnerID=40&md5=108a2ed2d5b9629088398271ec87a050
Endophytic fungi of the medicinal herb, Ocimum sanctum are believed to possess antifungal activity against pathogenic fungi. Due to the emergence of pathogenic fungi and antibiotic-resistant strains, the search for alternative antimicrobial agents is a need. The present study aimed to evaluate the antifungal activity of endophytic fungi isolated from O. sanctum. Plate-to-plate method, disk diffusion assay, and Scanning electron microscopic (SEM) were employed in this study. The finding revealed that fungal isolates Colletotrichum sp. IBRL OS-39, Aspergillus sp. IBRL OS-65, Muscodor sp. IBRL OS-94 and Muscodor sp. IBRL OS-98 was able to produce volatile compounds with antifungal activity against pathogenic fungi. On the disk diffusion assay, Lasiodiplodia sp. IBRL OS-64, and Muscodor sp. IBRL OS-94 displayed good antifungal activity against test fungi with a diameter of inhibition zone between 9.6±0.6-14.3±0.6 mm and 11.2±1.2-15.7±0.6 mm, respectively. SEM observations revealed remarkable morphological changes in Candida albicans treated with the dichloromethane extracts of Lasiodiplodia sp. IBRL OS-64 and Muscodor sp. IBRL OS-94 with severe cell damage beyond repair and thus leads to cell death. © 2024, Penerbit UTM Press. All rights reserved.
Penerbit UTM Press
1279696
English
Article
All Open Access; Gold Open Access
author Jalil M.T.M.; Yahya M.F.Z.R.; Zakaria N.A.; Ibrahim D.
spellingShingle Jalil M.T.M.; Yahya M.F.Z.R.; Zakaria N.A.; Ibrahim D.
ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
author_facet Jalil M.T.M.; Yahya M.F.Z.R.; Zakaria N.A.; Ibrahim D.
author_sort Jalil M.T.M.; Yahya M.F.Z.R.; Zakaria N.A.; Ibrahim D.
title ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_short ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_full ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_fullStr ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_full_unstemmed ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
title_sort ANTIFUNGAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH OCIMUM SANCTUM
publishDate 2024
container_title Jurnal Teknologi
container_volume 86
container_issue 2
doi_str_mv 10.11113/jurnalteknologi.v86.20508
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185653830&doi=10.11113%2fjurnalteknologi.v86.20508&partnerID=40&md5=108a2ed2d5b9629088398271ec87a050
description Endophytic fungi of the medicinal herb, Ocimum sanctum are believed to possess antifungal activity against pathogenic fungi. Due to the emergence of pathogenic fungi and antibiotic-resistant strains, the search for alternative antimicrobial agents is a need. The present study aimed to evaluate the antifungal activity of endophytic fungi isolated from O. sanctum. Plate-to-plate method, disk diffusion assay, and Scanning electron microscopic (SEM) were employed in this study. The finding revealed that fungal isolates Colletotrichum sp. IBRL OS-39, Aspergillus sp. IBRL OS-65, Muscodor sp. IBRL OS-94 and Muscodor sp. IBRL OS-98 was able to produce volatile compounds with antifungal activity against pathogenic fungi. On the disk diffusion assay, Lasiodiplodia sp. IBRL OS-64, and Muscodor sp. IBRL OS-94 displayed good antifungal activity against test fungi with a diameter of inhibition zone between 9.6±0.6-14.3±0.6 mm and 11.2±1.2-15.7±0.6 mm, respectively. SEM observations revealed remarkable morphological changes in Candida albicans treated with the dichloromethane extracts of Lasiodiplodia sp. IBRL OS-64 and Muscodor sp. IBRL OS-94 with severe cell damage beyond repair and thus leads to cell death. © 2024, Penerbit UTM Press. All rights reserved.
publisher Penerbit UTM Press
issn 1279696
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
_version_ 1809677882830618624