GENETIC TYPING OF THREE MALAYSIAN DURIAN VARIETIES USING SIMPLE SEQUENCE REPEAT (SSR) MARKERS

• Published in: Malaysian Journal of Biochemistry and Molecular Biology
• Main Author: Ismail A.M.; Hassan W.R.M.; Razak A.R.A.; Yusof F.Z.M.
• Format: Article
• Language: English
• Published: Malaysian Society for Biochemistry and Molecular Biology 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185571563&partnerID=40&md5=e6b1c9057b4251dc23d6285b9e7ed8a0

There have been few studies on the molecular aspects of durian up to this point. Although morphological classification is practical, simple, and quick, it suffers from phenotypic plasticity due to environmental influences and age. Hence, the genetic characterization of durian varieties needs to be carried out. This study was performed to evaluate the effectiveness of three simple sequence repeat (SSR) markers to identify and discriminate three durian types: MK (Musang King), D24 and D101. Successful amplification of SSR regions were observed in the durian DNA samples. A total of 8 alleles were generated by all primers. Both DZ01 and DZ04 primers showed the highest number of polymorphic fragments. Among the three SSR primers, DZ03 is the least informative with only 2 number of alleles produced. The most important finding of this study was that each primer is unique and specific to one type of durian sample. This preliminary study showed that the SSR loci could be used as genetic markers to assist future durian breeding program. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.