Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs

Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclu...

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Published in:Scientific Reports
Main Author: Dinter C.; Gumprecht A.; Menze M.A.; Azizan A.; Niehoff P.-J.; Hansen S.; Büchs J.
Format: Article
Language:English
Published: Nature Research 2024
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185102685&doi=10.1038%2fs41598-024-53980-7&partnerID=40&md5=87ed8f265c549992bab31100d948c825
id 2-s2.0-85185102685
spelling 2-s2.0-85185102685
Dinter C.; Gumprecht A.; Menze M.A.; Azizan A.; Niehoff P.-J.; Hansen S.; Büchs J.
Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
2024
Scientific Reports
14
1
10.1038/s41598-024-53980-7
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185102685&doi=10.1038%2fs41598-024-53980-7&partnerID=40&md5=87ed8f265c549992bab31100d948c825
Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclusively concentrated on fluids at waterlike viscosity. The importance of CFD model validation cannot be overstated. While techniques to elucidate the internal flow field are necessary for model validation in STRs, the liquid distribution, caused by the orbital shaking motion of shake flasks, can be exploited for model validation. An OpenFOAM CFD model for shake flasks has been established. Calculated liquid distributions were compared to suitable, previously published experimental data. Across a broad range of shaking conditions, at waterlike and moderate viscosity (16.7 mPa∙s), the CFD model's liquid distributions align excellently with the experimental data, in terms of overall shape and position of the liquid relative to the direction of the centrifugal force. Additionally, the CFD model was used to calculate the volumetric power input, based on the energy dissipation. Depending on the shaking conditions, the computed volumetric power inputs range from 0.1 to 7 kW/m3 and differed on average by 0.01 kW/m3 from measured literature data. © The Author(s) 2024.
Nature Research
20452322
English
Article
All Open Access; Gold Open Access
author Dinter C.; Gumprecht A.; Menze M.A.; Azizan A.; Niehoff P.-J.; Hansen S.; Büchs J.
spellingShingle Dinter C.; Gumprecht A.; Menze M.A.; Azizan A.; Niehoff P.-J.; Hansen S.; Büchs J.
Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
author_facet Dinter C.; Gumprecht A.; Menze M.A.; Azizan A.; Niehoff P.-J.; Hansen S.; Büchs J.
author_sort Dinter C.; Gumprecht A.; Menze M.A.; Azizan A.; Niehoff P.-J.; Hansen S.; Büchs J.
title Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_short Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_full Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_fullStr Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_full_unstemmed Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
title_sort Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs
publishDate 2024
container_title Scientific Reports
container_volume 14
container_issue 1
doi_str_mv 10.1038/s41598-024-53980-7
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185102685&doi=10.1038%2fs41598-024-53980-7&partnerID=40&md5=87ed8f265c549992bab31100d948c825
description Computational fluid dynamics (CFD) has recently become a pivotal tool in the design and scale-up of bioprocesses. While CFD has been extensively utilized for stirred tank reactors (STRs), there exists a relatively limited body of literature focusing on CFD applications for shake flasks, almost exclusively concentrated on fluids at waterlike viscosity. The importance of CFD model validation cannot be overstated. While techniques to elucidate the internal flow field are necessary for model validation in STRs, the liquid distribution, caused by the orbital shaking motion of shake flasks, can be exploited for model validation. An OpenFOAM CFD model for shake flasks has been established. Calculated liquid distributions were compared to suitable, previously published experimental data. Across a broad range of shaking conditions, at waterlike and moderate viscosity (16.7 mPa∙s), the CFD model's liquid distributions align excellently with the experimental data, in terms of overall shape and position of the liquid relative to the direction of the centrifugal force. Additionally, the CFD model was used to calculate the volumetric power input, based on the energy dissipation. Depending on the shaking conditions, the computed volumetric power inputs range from 0.1 to 7 kW/m3 and differed on average by 0.01 kW/m3 from measured literature data. © The Author(s) 2024.
publisher Nature Research
issn 20452322
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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