| Summary: | Diabetes Mellitus (DM) is still one of the world's major health problems, contributing to many complications leading to increased morbidity and mortality. Different approaches have been performed to enhance the patient's quality of life. Over the past decade, due to a trend in herbal medicines, numerous plants, including Mareme and Sala plants, have been claimed to be advantageous in alleviating DM features in pre-clinical research. Mareme and Sala leaves contain flavonoid compounds, which are potential as antidiabetic agents. This study aimed to determine the in vitro inhibitory activity of the ethanolic extracts of both plants on the αglucosidase enzyme that plays a pivotal role in diabetic pathophysiology. The inhibition potential of the combination of Mareme and Sala leaf ethanol extracts and the positive control, namely acarbose, were measured using an ELISA reader at 450 nm based on the formation of p-nitrophenol. The ELISA reader measurements showed that the combination of Mareme leaves and Sala leaf ethanol extract could not inhibit α glucosidase activity in vitro, indicated by the IC50 calculation of 374.47 ppm. Nevertheless, our research revealed the presence of diverse secondary metabolites, including flavonoids, in both extracts, which could potentially be responsible for further pharmacological activities. © 2019 ANGIOTHERAPY, a publication of Eman Research Ltd, Australia.
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