Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies

Heparin, a sulfated glycosaminogylcan (GAG) is one of macromolecule natural compounds and widely used as an anticoagulant drug, anti-thrombotic agent and hemodialysis (patients undergoing kidney dialysis). Since it is derived from animal source, heparin can contain several natural contaminants. Thus...

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Published in:Proceedings of International Conference on Research in Education and Science
Main Author: Nasir A.M.; Ishak N.; Anuar A.; Abdullah S.
Format: Conference paper
Language:English
Published: The International Society for Technology Education and Science 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184331604&partnerID=40&md5=0c1ab10a7a9d0b5ee7a302c91a591543
id 2-s2.0-85184331604
spelling 2-s2.0-85184331604
Nasir A.M.; Ishak N.; Anuar A.; Abdullah S.
Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
2023
Proceedings of International Conference on Research in Education and Science
9
1

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184331604&partnerID=40&md5=0c1ab10a7a9d0b5ee7a302c91a591543
Heparin, a sulfated glycosaminogylcan (GAG) is one of macromolecule natural compounds and widely used as an anticoagulant drug, anti-thrombotic agent and hemodialysis (patients undergoing kidney dialysis). Since it is derived from animal source, heparin can contain several natural contaminants. Thus, molecular imprinting technology is introduced for purification and separation of heparin. An epitope extraction was implemented consist of low molecular weight heparin (LMWH) as template to capture large molecule of heparin which prepared by sol-gel process on the surface of macromolecule silica using 3-aminopropyltriethoxysilane (APTES) and tetraethoxysilane (TEOS) and functional monomer and cross-linker respectively. Here, the effect of template: monomer ratio was evaluated and result shown that the (1:2) ratio of template to functional monomer successfully adsorb and enrich the heparin protein. The imprinted polymer was characterized with Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetric Analysis (TGA), Brunauer-Emmet-Teller (BET) and Field Emission Scanning Electron Microscopy (FESEM). The adsorption behaviours of epitope imprinting indicated that Langmuir-Freundlich (single-site) was considered as the model with better fit and kinetic batch studies showed pseudo-first-order kinetic model via physicochemical process. The results obtained good imprinting comparable of epitope imprinting effect. © 2023 Published by the ISTES Organization
The International Society for Technology Education and Science
28336747
English
Conference paper

author Nasir A.M.; Ishak N.; Anuar A.; Abdullah S.
spellingShingle Nasir A.M.; Ishak N.; Anuar A.; Abdullah S.
Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
author_facet Nasir A.M.; Ishak N.; Anuar A.; Abdullah S.
author_sort Nasir A.M.; Ishak N.; Anuar A.; Abdullah S.
title Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
title_short Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
title_full Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
title_fullStr Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
title_full_unstemmed Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
title_sort Heparin Epitope Imprinted Via Sol-Gel Process on Silica Surface: Effect of Template: Monomer Ratio Studies
publishDate 2023
container_title Proceedings of International Conference on Research in Education and Science
container_volume 9
container_issue 1
doi_str_mv
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184331604&partnerID=40&md5=0c1ab10a7a9d0b5ee7a302c91a591543
description Heparin, a sulfated glycosaminogylcan (GAG) is one of macromolecule natural compounds and widely used as an anticoagulant drug, anti-thrombotic agent and hemodialysis (patients undergoing kidney dialysis). Since it is derived from animal source, heparin can contain several natural contaminants. Thus, molecular imprinting technology is introduced for purification and separation of heparin. An epitope extraction was implemented consist of low molecular weight heparin (LMWH) as template to capture large molecule of heparin which prepared by sol-gel process on the surface of macromolecule silica using 3-aminopropyltriethoxysilane (APTES) and tetraethoxysilane (TEOS) and functional monomer and cross-linker respectively. Here, the effect of template: monomer ratio was evaluated and result shown that the (1:2) ratio of template to functional monomer successfully adsorb and enrich the heparin protein. The imprinted polymer was characterized with Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetric Analysis (TGA), Brunauer-Emmet-Teller (BET) and Field Emission Scanning Electron Microscopy (FESEM). The adsorption behaviours of epitope imprinting indicated that Langmuir-Freundlich (single-site) was considered as the model with better fit and kinetic batch studies showed pseudo-first-order kinetic model via physicochemical process. The results obtained good imprinting comparable of epitope imprinting effect. © 2023 Published by the ISTES Organization
publisher The International Society for Technology Education and Science
issn 28336747
language English
format Conference paper
accesstype
record_format scopus
collection Scopus
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