BENZIMIDAZOLE DERIVATIVES AS POTENTIAL NEURAMINIDASE INHIBITORS: CONVENTIONAL AND MICROWAVE SYNTHESIS, In Vitro AND MOLECULAR DOCKING ANALYSIS; [Terbitan Benzimidazola yang Berpotensi sebagai Perencat Neuraminidase: Sintesis secara Konvensional dan Gelombang Mikro, Analisis In Vitro dan Pengedokan Molekul]

• Published in: Malaysian Journal of Analytical Sciences
• Main Author: Hamzah N.; Hamid S.A.; Rahim A.S.A.; Wahab H.A.
• Format: Article
• Language: English
• Published: Malaysian Society of Analytical Sciences 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183842212&partnerID=40&md5=a4153064d3f5f4eb8ed16420960f6230

As a continuous effort to discover potential neuraminidase (NA) inhibitors, two series of benzimidazole derivatives consisting of esters, 5(a–g) and carboxylic acid moieties, 6(a–g) were synthesised under conventional and microwave conditions. The efficiency of both methods was compared, and their ability to inhibit the action of NA enzyme was examined in silico and in vitro. The microwave synthesis of the target compounds was more efficient and convenient than the conventional method as the former accelerated the reaction from hours to minutes, giving comparable yields. All compounds obtained were confirmed by the1H,13C NMR, and mass spectroscopic data. Out of six compounds tested in the carboxylic acid series, only 6f showed inhibitory action towards NA with 15.2%. The binding interactions of 6(a–g) were investigated further by molecular docking on the NA active site (PDB ID: 3TI6). 6f was found to interact in the 430-loop cavity mainly by hydrophobic interactions. 6f interacted at different active sites compared to DANA and oseltamivir. Although the compounds showed low inhibitory action, with strategic structural improvements, the benzimidazoles have the potential to be developed as NA inhibitors. © 2023, Malaysian Society of Analytical Sciences. All rights reserved.