Different Enzymatic Strategy to Degrade Carbamazepine by Rhodococcus zopfii and Leifsonia shinshuensis

• Published in: Proceedings of the National Academy of Sciences India Section B - Biological Sciences
• Main Author: Tay C.C.; Mohamad-Nasir N.; Hashim S.N.; Lokman N.F.; Wong K.K.
• Format: Article
• Language: English
• Published: Springer 2024
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85182675180&doi=10.1007%2fs40011-023-01539-3&partnerID=40&md5=912c99e6db1c421130d0ce75bce02939

This research reports on the efficiency of Rhodococcus zopfii and Leifsonia shinshuensis to degrade approximately 99.98 ± 0.20% carbamazepine (CBZ) within 5 days. The degradation of CBZ was strongly correlated (R2 ≥ 0.9) to bacterial growth in L. shinshuensis, but only moderate (R2 ≈ 0.6) in R. zopfii. Both oxygen oxidoreductase and aldehyde oxidase concentrations were positively and strongly correlated (R2 ≈ 0.9) to the degradation rate of CBZ in both cultures. However, when it comes to bacterial growth (CFU mL−1/h), only the culture of L. shinshuensis showed a strong positive correlation (R2 ≈ 0.9) to both enzyme concentrations, whereas the culture of R. zopfii only shows a moderate correlation (R2 ≈ 0.7). The degradation of CBZ by R. zopfii did not directly translate into higher bacterial growth. This demonstrated the possibility of different CBZ degradation strategies was being adopted by these two different bacteria. Hence, this piece of information is important to elucidate the mechanisms of carbamazepine degradation and sustainable treatment. © The Author(s), under exclusive licence to The National Academy of Sciences, India 2024.