| Summary: | Some microorganisms with the potential to cause significant diseases are becoming resistant to the majority of currently available antibiotics. This research aims to extract bioactive compounds with antimicrobial properties from Etlingera coccinea using methanol, and to assess the antimicrobial effectiveness of E. coccinea against various pathogenic microorganisms such as Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus), Klebsiella pneumoniae (K. pneumoniae), Shigella sonnei (S. sonnei), Candida albicans (C. albicans), and Saccharomyces cerevisiae (S. cerevisiae) at different concentration levels (25%, 50%, 75%, and 100%). The experiment employed the maceration method to extract antimicrobial compounds from E. coccinea leaves, and the antimicrobial activity was assessed using the Kirby-Bauer Disc Diffusion assay. The outcomes revealed that the raw extract of E. coccinea effectively hindered the growth of all six pathogenic microorganisms within the concentration range of 25% to 100%, with the largest inhibition zone observed for B. cereus (13.83mm) and the smallest for S. cerevisiae (5.33mm), both at 100% concentration of E. coccinea crude extract. The results of the univariate analysis of variance (ANOVA) indicate a significant distinction (p<0.05) between the crude extract and the positive control substances employed in this study (Ampicillin, Vancomycin, Fluconazole). In conclusion, the findings of this investigation could have meaningful implications for the advancement of antimicrobial agents derived from native Malaysian plant sources. © 2024 Author(s).
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