PRECONCENTRATION AND DETERMINATION OF NICOTINE IN TOBACCO SAMPLES BY DISPERSIVE MICRO-SOLID PHASE EXTRACTION AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY; [Pemekatan dan Penentuan Nikotin dalam Sampel Tembakau oleh Pengekstrakan Fasa Pepejal-Mikro Dispersif dan Gas Kromatografi-Spektrometri Jisim]

• Published in: Malaysian Journal of Analytical Sciences
• Main Author: Yatim N.A.S.M.; Hanapi N.S.M.; Zaini N.; Anis A.L.; Rashid R.A.; Bonnia N.N.; Ibrahim W.N.W.
• Format: Article
• Language: English
• Published: Malaysian Society of Analytical Sciences 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181722740&partnerID=40&md5=b03a0db2f3969304163e46d69a4a7b64

In this study, the dispersive micro-solid phase extraction (D-µ-SPE) method was optimised and validated for preconcentration and determination of nicotine in tobacco samples, with the use of C18 as sorbent, coupled with gas chromatography-mass spectrometry (GC-MS). The optimisation of three extraction parameters, namely extraction time (min), mass of sorbent (mg), and desorption volume (mL), were investigated to provide better insight into its reliability as a low-consumption sorbent and rapid operation. The obtained parameters suitable for nicotine extraction were 10 min of extraction time, 50 mg of C18 sorbent, and 1.0 mL of desorption solvent. Nicotine was successfully extracted from tobacco samples with good linearity in the range of 0.1 – 500 mg/L, with a coefficient of determination (R2) of 0.9997. The calculated LOD resulted in a value of 0.0983 mg/L, with the calculated LOQ being 0.2978 mg/L. The precision of the method revealed a percent relative standard deviation (% RSD) of 2.6%. In addition, good relative recovery (%) of nicotine in real samples was also achieved in the 75.5 – 96.4% range, with % RSD ranging from 3.1-5.7%. Hence, this study proposed an improvement to the green method towards nicotine analysis with low consumption of organic solvent, good sorbent absorptivity, and rapid and high selectivity for separation. © 2023, Malaysian Society of Analytical Sciences. All rights reserved.