The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection

Acute hepatopancreatic necrosis disease (AHPND) has inflicted massive economic losses and posed a considerable threat to the development of penaeid shrimp aquaculture in Malaysia. The restriction on antibiotic uses have necessitated exploring alternatives preventive measures against AHPND outbreak,...

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Published in:Aquaculture Reports
Main Author: Chin Y.K.; Haifa-Haryani W.O.; Nazarudin M.F.; Ahmad M.I.; Azzam-Sayuti M.; Mohamad A.; Ida-Muryany M.Y.; Karim M.; Salleh A.; Amal M.N.A.; Norhariani M.N.; Ina-Salwany M.Y.
Format: Article
Language:English
Published: Elsevier B.V. 2024
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181681126&doi=10.1016%2fj.aqrep.2023.101903&partnerID=40&md5=1706be1c9cece6f732c32d5da0a3bb38
id 2-s2.0-85181681126
spelling 2-s2.0-85181681126
Chin Y.K.; Haifa-Haryani W.O.; Nazarudin M.F.; Ahmad M.I.; Azzam-Sayuti M.; Mohamad A.; Ida-Muryany M.Y.; Karim M.; Salleh A.; Amal M.N.A.; Norhariani M.N.; Ina-Salwany M.Y.
The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
2024
Aquaculture Reports
34

10.1016/j.aqrep.2023.101903
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181681126&doi=10.1016%2fj.aqrep.2023.101903&partnerID=40&md5=1706be1c9cece6f732c32d5da0a3bb38
Acute hepatopancreatic necrosis disease (AHPND) has inflicted massive economic losses and posed a considerable threat to the development of penaeid shrimp aquaculture in Malaysia. The restriction on antibiotic uses have necessitated exploring alternatives preventive measures against AHPND outbreak, such as the use beneficial bacteria and nutritional additives. In this study, diets which were fed to Penaeus monodon postlarvae (PL15) were fed with diets containing probiotic (L. plantarum at 108 CFU/mL), prebiotic (S. polycystum at 2%) and synbiotic diets (combination of probiotic and prebiotic) formulations. These diets were administered in triplicate for 35 days, followed by an immersion challenge with 6 × 105 CFU/mL AHPND-causing V. parahaemolyticus S2–4. Sampling of shrimp's cephalothorax and abdomen from the feeding trial and the immersion challenge were conducted for gene expression and histopathology analyses. Results indicated that the synbiotic-fed group displayed the most significant weight gain, specific growth rate, and protein conversion ratio among the tested groups. In addition, shrimp cephalothorax from this group displayed a significant immune response, with expression of LGBP, peroxinectin, prophenoloxidase during post-feeding trial, and expression of prophenoloxidase, toll-like receptor, penaeidin, during post-challenge trial. Furthermore, the highest L. plantarum concentration in abdomen, lowest V. parahaemolyticus S2–4 concentration in cephalothorax and highest percent survival of shrimp during post-challenge were observed in this group. Therefore, this study highlighted the positive effect of synbiotic-supplemented diet on growth, immune response, and disease resistance of shrimp, offering a promising and sustainable solution to alleviate substantial production losses in shrimp farming. © 2023 The Authors
Elsevier B.V.
23525134
English
Article
All Open Access; Gold Open Access
author Chin Y.K.; Haifa-Haryani W.O.; Nazarudin M.F.; Ahmad M.I.; Azzam-Sayuti M.; Mohamad A.; Ida-Muryany M.Y.; Karim M.; Salleh A.; Amal M.N.A.; Norhariani M.N.; Ina-Salwany M.Y.
spellingShingle Chin Y.K.; Haifa-Haryani W.O.; Nazarudin M.F.; Ahmad M.I.; Azzam-Sayuti M.; Mohamad A.; Ida-Muryany M.Y.; Karim M.; Salleh A.; Amal M.N.A.; Norhariani M.N.; Ina-Salwany M.Y.
The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
author_facet Chin Y.K.; Haifa-Haryani W.O.; Nazarudin M.F.; Ahmad M.I.; Azzam-Sayuti M.; Mohamad A.; Ida-Muryany M.Y.; Karim M.; Salleh A.; Amal M.N.A.; Norhariani M.N.; Ina-Salwany M.Y.
author_sort Chin Y.K.; Haifa-Haryani W.O.; Nazarudin M.F.; Ahmad M.I.; Azzam-Sayuti M.; Mohamad A.; Ida-Muryany M.Y.; Karim M.; Salleh A.; Amal M.N.A.; Norhariani M.N.; Ina-Salwany M.Y.
title The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
title_short The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
title_full The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
title_fullStr The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
title_full_unstemmed The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
title_sort The synergistic Lactobacillus plantarum L20 and Sargassum polycystum -added diet for improvement of Black tiger shrimp, Penaeus monodon ‘s growth, immune responses, bacterial profiles, and resistance against Vibrio parahaemolyticus associated Acute hepatopancreatic necrosis disease (AHPND) infection
publishDate 2024
container_title Aquaculture Reports
container_volume 34
container_issue
doi_str_mv 10.1016/j.aqrep.2023.101903
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85181681126&doi=10.1016%2fj.aqrep.2023.101903&partnerID=40&md5=1706be1c9cece6f732c32d5da0a3bb38
description Acute hepatopancreatic necrosis disease (AHPND) has inflicted massive economic losses and posed a considerable threat to the development of penaeid shrimp aquaculture in Malaysia. The restriction on antibiotic uses have necessitated exploring alternatives preventive measures against AHPND outbreak, such as the use beneficial bacteria and nutritional additives. In this study, diets which were fed to Penaeus monodon postlarvae (PL15) were fed with diets containing probiotic (L. plantarum at 108 CFU/mL), prebiotic (S. polycystum at 2%) and synbiotic diets (combination of probiotic and prebiotic) formulations. These diets were administered in triplicate for 35 days, followed by an immersion challenge with 6 × 105 CFU/mL AHPND-causing V. parahaemolyticus S2–4. Sampling of shrimp's cephalothorax and abdomen from the feeding trial and the immersion challenge were conducted for gene expression and histopathology analyses. Results indicated that the synbiotic-fed group displayed the most significant weight gain, specific growth rate, and protein conversion ratio among the tested groups. In addition, shrimp cephalothorax from this group displayed a significant immune response, with expression of LGBP, peroxinectin, prophenoloxidase during post-feeding trial, and expression of prophenoloxidase, toll-like receptor, penaeidin, during post-challenge trial. Furthermore, the highest L. plantarum concentration in abdomen, lowest V. parahaemolyticus S2–4 concentration in cephalothorax and highest percent survival of shrimp during post-challenge were observed in this group. Therefore, this study highlighted the positive effect of synbiotic-supplemented diet on growth, immune response, and disease resistance of shrimp, offering a promising and sustainable solution to alleviate substantial production losses in shrimp farming. © 2023 The Authors
publisher Elsevier B.V.
issn 23525134
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
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