Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Prote...

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Published in:Malaysian Applied Biology
Main Author: Roszelin S.A.M.; Hazbir N.A.M.; Jumali S.S.; Shakri T.; Isa N.M.
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173248492&doi=10.55230%2fmabjournal.v52i3.2705&partnerID=40&md5=f39d7e6265c0acecb5363f3177f236db
id 2-s2.0-85173248492
spelling 2-s2.0-85173248492
Roszelin S.A.M.; Hazbir N.A.M.; Jumali S.S.; Shakri T.; Isa N.M.
Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
2023
Malaysian Applied Biology
52
3
10.55230/mabjournal.v52i3.2705
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173248492&doi=10.55230%2fmabjournal.v52i3.2705&partnerID=40&md5=f39d7e6265c0acecb5363f3177f236db
Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Proteins (SAPs) have been shown to play a crucial role in plant adaptation to biotic and abiotic stressors. They are encoded by a family of genes that produce a zinc finger protein with A20 and/or AN1 domains at either their N or C-terminal ends. Therefore, this study focused on understanding the role of the Oryza sativa SAP gene family (OsSAPs) in response to drought and salinity stress. In-silico analysis revealed that most of the OsSAP family members were upregulated by stress; two highly inducible OsSAP genes were also upregulated in response to stress under a rice-specific background. To study gene function, an Arabidopsis transformation system was employed using three genotypes: Col-0 (wild type), overexpressed transgenic OsSAP8, and atsap2 T-DNA knockout mutant. Arabidopsis AtSAP2 gene, which is homologous to rice OsSAP8, was used as a comparison to the loss of function mutation in Arabidopsis. Morpho-physiological analysis showed that the atsap2 mutant displayed a sensitive phenotype to drought and salinity stress through low relative chlorophyll content and delayed inflorescence development and flowering as compared to Col-0 and transgenic OsSAP8. This suggests that the abolished atsap2 gene may contribute to reduced stress tolerance in plants. In contrast, transgenic OsSAP8 overexpression demonstrated tolerance to drought and salinity stress by maintaining relative chlorophyll content under both stress conditions, indirectly reflecting sustained photosynthetic machinery and stable photosynthetic rate. Further investigation, such as measuring the photosynthesis rate, is required to establish the correlation between chlorophyll data and photosynthesis activity. © 2023 Malaysian Society of Applied Biology.
Malaysian Society of Applied Biology
1268643
English
Article
All Open Access; Bronze Open Access; Green Open Access
author Roszelin S.A.M.; Hazbir N.A.M.; Jumali S.S.; Shakri T.; Isa N.M.
spellingShingle Roszelin S.A.M.; Hazbir N.A.M.; Jumali S.S.; Shakri T.; Isa N.M.
Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
author_facet Roszelin S.A.M.; Hazbir N.A.M.; Jumali S.S.; Shakri T.; Isa N.M.
author_sort Roszelin S.A.M.; Hazbir N.A.M.; Jumali S.S.; Shakri T.; Isa N.M.
title Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
title_short Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
title_full Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
title_fullStr Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
title_full_unstemmed Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
title_sort Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis
publishDate 2023
container_title Malaysian Applied Biology
container_volume 52
container_issue 3
doi_str_mv 10.55230/mabjournal.v52i3.2705
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85173248492&doi=10.55230%2fmabjournal.v52i3.2705&partnerID=40&md5=f39d7e6265c0acecb5363f3177f236db
description Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Proteins (SAPs) have been shown to play a crucial role in plant adaptation to biotic and abiotic stressors. They are encoded by a family of genes that produce a zinc finger protein with A20 and/or AN1 domains at either their N or C-terminal ends. Therefore, this study focused on understanding the role of the Oryza sativa SAP gene family (OsSAPs) in response to drought and salinity stress. In-silico analysis revealed that most of the OsSAP family members were upregulated by stress; two highly inducible OsSAP genes were also upregulated in response to stress under a rice-specific background. To study gene function, an Arabidopsis transformation system was employed using three genotypes: Col-0 (wild type), overexpressed transgenic OsSAP8, and atsap2 T-DNA knockout mutant. Arabidopsis AtSAP2 gene, which is homologous to rice OsSAP8, was used as a comparison to the loss of function mutation in Arabidopsis. Morpho-physiological analysis showed that the atsap2 mutant displayed a sensitive phenotype to drought and salinity stress through low relative chlorophyll content and delayed inflorescence development and flowering as compared to Col-0 and transgenic OsSAP8. This suggests that the abolished atsap2 gene may contribute to reduced stress tolerance in plants. In contrast, transgenic OsSAP8 overexpression demonstrated tolerance to drought and salinity stress by maintaining relative chlorophyll content under both stress conditions, indirectly reflecting sustained photosynthetic machinery and stable photosynthetic rate. Further investigation, such as measuring the photosynthesis rate, is required to establish the correlation between chlorophyll data and photosynthesis activity. © 2023 Malaysian Society of Applied Biology.
publisher Malaysian Society of Applied Biology
issn 1268643
language English
format Article
accesstype All Open Access; Bronze Open Access; Green Open Access
record_format scopus
collection Scopus
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