Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)

Cabbage is a heat-stress-sensitive cool-season crop. When exposed to high temperatures, cabbage plants can experience reduced growth, wilting, leaf yellowing, and premature bolting (the formation of a flowering stem). The regulatory mechanism controlling heat stress is poorly understood in cabbage....

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Published in:Horticulturae
Main Author: Li Q.; Zhang G.; Zhao Y.; Gu L.; Wang Y.; Yu X.; Abdullah S.
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85172799913&doi=10.3390%2fhorticulturae9090977&partnerID=40&md5=20fa5bc3332507854f6f74fdd14fdcbe
id 2-s2.0-85172799913
spelling 2-s2.0-85172799913
Li Q.; Zhang G.; Zhao Y.; Gu L.; Wang Y.; Yu X.; Abdullah S.
Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
2023
Horticulturae
9
9
10.3390/horticulturae9090977
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85172799913&doi=10.3390%2fhorticulturae9090977&partnerID=40&md5=20fa5bc3332507854f6f74fdd14fdcbe
Cabbage is a heat-stress-sensitive cool-season crop. When exposed to high temperatures, cabbage plants can experience reduced growth, wilting, leaf yellowing, and premature bolting (the formation of a flowering stem). The regulatory mechanism controlling heat stress is poorly understood in cabbage. To investigate this mechanism, physiological changes and transcriptional profiling for different heat treatment times were analyzed in this study. The results showed that superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were enhanced under heat stress. In particular, the increase in SOD and POD activities after 12 h of heat treatment was greater than that after 4 h of heat treatment. With increasing heat treatment duration, the leaf CAT activity and H2O2 content decreased after the initial increase. The electrolyte leakage and malondialdehyde (MDA) content dropped significantly, while the proline content increased. Alongside that, 7007 and 5537 upregulated genes were identified in the experimental group treated with heat stress for the 4 h and 12 h treatments, respectively. We found that 10,479 DEGs were shared in the heat stress treatment, of which 1241 were associated with the heat treatment time. By integrating the expression patterns and functional annotations of genes related to heat stress, we identified 15 hub genes that respond to heat stress in cabbage. Meanwhile, we had constructed a physiological to molecular model of cabbage response to long-term heat stress. These findings provide new insights for the comprehensive analysis of cabbage response to heat stress and genetic resources for breeding new varieties of cabbage with heat tolerance via genetic engineering. © 2023 by the authors.
Multidisciplinary Digital Publishing Institute (MDPI)
23117524
English
Article
All Open Access; Gold Open Access
author Li Q.; Zhang G.; Zhao Y.; Gu L.; Wang Y.; Yu X.; Abdullah S.
spellingShingle Li Q.; Zhang G.; Zhao Y.; Gu L.; Wang Y.; Yu X.; Abdullah S.
Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
author_facet Li Q.; Zhang G.; Zhao Y.; Gu L.; Wang Y.; Yu X.; Abdullah S.
author_sort Li Q.; Zhang G.; Zhao Y.; Gu L.; Wang Y.; Yu X.; Abdullah S.
title Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
title_short Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
title_full Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
title_fullStr Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
title_full_unstemmed Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
title_sort Hub Gene Identification and Heat-Stress-Related Transcriptional Regulation Mechanism in Cabbage (Brassica oleracea L.)
publishDate 2023
container_title Horticulturae
container_volume 9
container_issue 9
doi_str_mv 10.3390/horticulturae9090977
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85172799913&doi=10.3390%2fhorticulturae9090977&partnerID=40&md5=20fa5bc3332507854f6f74fdd14fdcbe
description Cabbage is a heat-stress-sensitive cool-season crop. When exposed to high temperatures, cabbage plants can experience reduced growth, wilting, leaf yellowing, and premature bolting (the formation of a flowering stem). The regulatory mechanism controlling heat stress is poorly understood in cabbage. To investigate this mechanism, physiological changes and transcriptional profiling for different heat treatment times were analyzed in this study. The results showed that superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were enhanced under heat stress. In particular, the increase in SOD and POD activities after 12 h of heat treatment was greater than that after 4 h of heat treatment. With increasing heat treatment duration, the leaf CAT activity and H2O2 content decreased after the initial increase. The electrolyte leakage and malondialdehyde (MDA) content dropped significantly, while the proline content increased. Alongside that, 7007 and 5537 upregulated genes were identified in the experimental group treated with heat stress for the 4 h and 12 h treatments, respectively. We found that 10,479 DEGs were shared in the heat stress treatment, of which 1241 were associated with the heat treatment time. By integrating the expression patterns and functional annotations of genes related to heat stress, we identified 15 hub genes that respond to heat stress in cabbage. Meanwhile, we had constructed a physiological to molecular model of cabbage response to long-term heat stress. These findings provide new insights for the comprehensive analysis of cabbage response to heat stress and genetic resources for breeding new varieties of cabbage with heat tolerance via genetic engineering. © 2023 by the authors.
publisher Multidisciplinary Digital Publishing Institute (MDPI)
issn 23117524
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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