IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS

Dental plaque on tooth surfaces significantly contributes to dental caries leading to acidic demineralization of the tooth enamel and dentin. This is mainly caused by the formation of pathogenic bacterial biofilm. Dental caries affects approximately 3.5 million people around the world. Compared to s...

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Published in:Journal of Health and Translational Medicine
Main Author: Johari A.S.; Tarmizi N.H.; Zulkepli N.A.; Mokhtar N.; Ya’kub M.K.; Sarmoko S.
Format: Article
Language:English
Published: Faculty of Medicine, University of Malaya 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85172701986&doi=10.22452%2fjummec.sp2023no2.25&partnerID=40&md5=1bbc316f7eac362588eff175fd740e0b
id 2-s2.0-85172701986
spelling 2-s2.0-85172701986
Johari A.S.; Tarmizi N.H.; Zulkepli N.A.; Mokhtar N.; Ya’kub M.K.; Sarmoko S.
IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
2023
Journal of Health and Translational Medicine
26
Special Issue 2
10.22452/jummec.sp2023no2.25
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85172701986&doi=10.22452%2fjummec.sp2023no2.25&partnerID=40&md5=1bbc316f7eac362588eff175fd740e0b
Dental plaque on tooth surfaces significantly contributes to dental caries leading to acidic demineralization of the tooth enamel and dentin. This is mainly caused by the formation of pathogenic bacterial biofilm. Dental caries affects approximately 3.5 million people around the world. Compared to synthetic anticariogenic agents such as chlorohexidine, plant-based compounds were found to have fewer side effects and higher economic value. Pineapple (Ananas comosus) is one of the largely commercialized plants in Malaysia with beneficial attributes such as its Bromelain enzyme. This study aimed to purify Bromelain enzyme from MD2 pineapple core and to evaluate their antibacterial properties against dental caries-associate pathogens in vitro. This study evaluated the effect of Bromelain enzyme isolated from MD2 pineapple core using 50% ammonium sulphate precipitation incorporated with centrifugal ultrafiltration against Streptococcus mutans, Staphylococcus aureus, Lactobacillus acidophilus, and Lactobacillus casei. Bromelain enzyme showed effectivity of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for all cariogenic organisms. Ultrafiltrate Bromelain (UFB) enzyme had significant antibacterial activity against S. mutans with a MIC value of 250 μg/ml and complete susceptibility at an MBC value of 1000 μg/ml. Biofilm inhibition against S. mutans increased from 45.98% to 57.48% with ultrafiltration. A 3.25 purification fold increase was observed in the ultrafiltrate sample. In conclusion, the ultrafiltration technique is efficient in purifying Bromelain enzyme and effectively inhibits S. mutans biofilm formation and thus possesses a potential inhibitory effect on dental caries in vitro. © 2023, Faculty of Medicine, University of Malaya. All rights reserved.
Faculty of Medicine, University of Malaya
18237339
English
Article

author Johari A.S.; Tarmizi N.H.; Zulkepli N.A.; Mokhtar N.; Ya’kub M.K.; Sarmoko S.
spellingShingle Johari A.S.; Tarmizi N.H.; Zulkepli N.A.; Mokhtar N.; Ya’kub M.K.; Sarmoko S.
IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
author_facet Johari A.S.; Tarmizi N.H.; Zulkepli N.A.; Mokhtar N.; Ya’kub M.K.; Sarmoko S.
author_sort Johari A.S.; Tarmizi N.H.; Zulkepli N.A.; Mokhtar N.; Ya’kub M.K.; Sarmoko S.
title IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
title_short IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
title_full IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
title_fullStr IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
title_full_unstemmed IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
title_sort IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF ULTRAFILTRATED-BROMELAIN AGAINST DENTAL CARIES PATHOGENS
publishDate 2023
container_title Journal of Health and Translational Medicine
container_volume 26
container_issue Special Issue 2
doi_str_mv 10.22452/jummec.sp2023no2.25
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85172701986&doi=10.22452%2fjummec.sp2023no2.25&partnerID=40&md5=1bbc316f7eac362588eff175fd740e0b
description Dental plaque on tooth surfaces significantly contributes to dental caries leading to acidic demineralization of the tooth enamel and dentin. This is mainly caused by the formation of pathogenic bacterial biofilm. Dental caries affects approximately 3.5 million people around the world. Compared to synthetic anticariogenic agents such as chlorohexidine, plant-based compounds were found to have fewer side effects and higher economic value. Pineapple (Ananas comosus) is one of the largely commercialized plants in Malaysia with beneficial attributes such as its Bromelain enzyme. This study aimed to purify Bromelain enzyme from MD2 pineapple core and to evaluate their antibacterial properties against dental caries-associate pathogens in vitro. This study evaluated the effect of Bromelain enzyme isolated from MD2 pineapple core using 50% ammonium sulphate precipitation incorporated with centrifugal ultrafiltration against Streptococcus mutans, Staphylococcus aureus, Lactobacillus acidophilus, and Lactobacillus casei. Bromelain enzyme showed effectivity of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for all cariogenic organisms. Ultrafiltrate Bromelain (UFB) enzyme had significant antibacterial activity against S. mutans with a MIC value of 250 μg/ml and complete susceptibility at an MBC value of 1000 μg/ml. Biofilm inhibition against S. mutans increased from 45.98% to 57.48% with ultrafiltration. A 3.25 purification fold increase was observed in the ultrafiltrate sample. In conclusion, the ultrafiltration technique is efficient in purifying Bromelain enzyme and effectively inhibits S. mutans biofilm formation and thus possesses a potential inhibitory effect on dental caries in vitro. © 2023, Faculty of Medicine, University of Malaya. All rights reserved.
publisher Faculty of Medicine, University of Malaya
issn 18237339
language English
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