Generation of a novel ex-vivo model to study re-endothelialization

• Published in: Artificial Cells, Nanomedicine and Biotechnology
• Main Author: Azman S.S.; Yazid M.D.; Abdul Ghani N.A.; Raja Sabudin R.Z.A.; Abdul Rahman M.R.; Sulaiman N.
• Format: Article
• Language: English
• Published: Taylor and Francis Ltd. 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85168061957&doi=10.1080%2f21691401.2023.2245456&partnerID=40&md5=4feedd86bae0587c6fa1fc1ef3ff5156

Endothelial dysfunction initiates the pathogenesis of a myriad of cardiovascular diseases, yet the precise underlying mechanisms remain unclear. Current model utilises mechanical denudation of arteries resulting in an arterial-injury model with onset of intimal hyperplasia (IH). Our study shows that 5 min enzymatic denudation of human umbilical artery (hUA) lumen at 37 °C efficiently denudes hUA while maintaining vessel integrity without significantly increase intima-media thickness after 7 days in culture. This ex-vivo model will be a valuable tool in understanding the mechanism of re-endothelialization prior to smooth muscle cells (SMC) activation thus placating IH at an early stage. © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.