Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques

Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques

The human amniotic fluid (AF) at full-term gestation has been observed to contain highly potent stem cells. Several works on human full-term amniotic fluid stem cells (AFSCs) have categorized the cells as broad multipotent and nontumorigenic upon transplantation. Being a merely discarded fluid, proc...

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Bibliographic Details
Published in:Stem Cell Laboratory Techniques: A Guide for Researchers and Students
Main Author: Nordin N.; Abdul Rahman K.A.; Ferdaos N.; Hamid A.A.; Joharry M.K.; Rejali Z.; Karuppiah T.; Yazid M.N.
Format: Book chapter
Language:English
Published: Elsevier 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85166565372&doi=10.1016%2fB978-0-12-823729-8.00004-X&partnerID=40&md5=ce791becfc27cd7a3c1969a0433319ce
id 2-s2.0-85166565372
spelling 2-s2.0-85166565372
Nordin N.; Abdul Rahman K.A.; Ferdaos N.; Hamid A.A.; Joharry M.K.; Rejali Z.; Karuppiah T.; Yazid M.N.
Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
2023
Stem Cell Laboratory Techniques: A Guide for Researchers and Students


10.1016/B978-0-12-823729-8.00004-X
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85166565372&doi=10.1016%2fB978-0-12-823729-8.00004-X&partnerID=40&md5=ce791becfc27cd7a3c1969a0433319ce
The human amniotic fluid (AF) at full-term gestation has been observed to contain highly potent stem cells. Several works on human full-term amniotic fluid stem cells (AFSCs) have categorized the cells as broad multipotent and nontumorigenic upon transplantation. Being a merely discarded fluid, procurement of the full-term AF imposes no risk to the baby and the mother and therefore involves less ethical concerns. These cells express several pluripotency markers, such as Oct-4, Sox2, Nanog, and SSEA, and differentiate into cells of the three primary germ layers, including neural progenitors. These characteristics have put them to become one of the prospective cells for transplantation. This chapter aims to describe the establishment and the characteristics of AFSCs from the human AF at full-term gestation. © 2023 Elsevier Inc. All rights reserved.
Elsevier

English
Book chapter
author Nordin N.; Abdul Rahman K.A.; Ferdaos N.; Hamid A.A.; Joharry M.K.; Rejali Z.; Karuppiah T.; Yazid M.N.
spellingShingle Nordin N.; Abdul Rahman K.A.; Ferdaos N.; Hamid A.A.; Joharry M.K.; Rejali Z.; Karuppiah T.; Yazid M.N.
Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
author_facet Nordin N.; Abdul Rahman K.A.; Ferdaos N.; Hamid A.A.; Joharry M.K.; Rejali Z.; Karuppiah T.; Yazid M.N.
author_sort Nordin N.; Abdul Rahman K.A.; Ferdaos N.; Hamid A.A.; Joharry M.K.; Rejali Z.; Karuppiah T.; Yazid M.N.
title Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
title_short Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
title_full Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
title_fullStr Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
title_full_unstemmed Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
title_sort Isolation of human stem cells from full-term gestation amniotic fluid using immunoselection and one-stage culture techniques
publishDate 2023
container_title Stem Cell Laboratory Techniques: A Guide for Researchers and Students
container_volume
container_issue
doi_str_mv 10.1016/B978-0-12-823729-8.00004-X
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85166565372&doi=10.1016%2fB978-0-12-823729-8.00004-X&partnerID=40&md5=ce791becfc27cd7a3c1969a0433319ce
description The human amniotic fluid (AF) at full-term gestation has been observed to contain highly potent stem cells. Several works on human full-term amniotic fluid stem cells (AFSCs) have categorized the cells as broad multipotent and nontumorigenic upon transplantation. Being a merely discarded fluid, procurement of the full-term AF imposes no risk to the baby and the mother and therefore involves less ethical concerns. These cells express several pluripotency markers, such as Oct-4, Sox2, Nanog, and SSEA, and differentiate into cells of the three primary germ layers, including neural progenitors. These characteristics have put them to become one of the prospective cells for transplantation. This chapter aims to describe the establishment and the characteristics of AFSCs from the human AF at full-term gestation. © 2023 Elsevier Inc. All rights reserved.
publisher Elsevier
issn
language English
format Book chapter
accesstype
record_format scopus
collection Scopus
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