Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens

Gram-positive pathogens cause infections such as pneumonia, skin infections, anthrax, and sinusitis. The objective of this study was to determine the phytochemical profile, antibacterial and antibiofilm efficacy of Swietenia macrophylla methanolic extract (SMME) against Gram-positive pathogens. The...

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Published in:Malaysian Applied Biology
Main Author: Amran S.S.D.; Jalil M.T.M.; Aziz A.A.; Yahya M.F.Z.R.
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164167532&doi=10.55230%2fmabjournal.v52i2.2642&partnerID=40&md5=df2b35266546e484108b8ca0d7414257
id 2-s2.0-85164167532
spelling 2-s2.0-85164167532
Amran S.S.D.; Jalil M.T.M.; Aziz A.A.; Yahya M.F.Z.R.
Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
2023
Malaysian Applied Biology
52
2
10.55230/mabjournal.v52i2.2642
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164167532&doi=10.55230%2fmabjournal.v52i2.2642&partnerID=40&md5=df2b35266546e484108b8ca0d7414257
Gram-positive pathogens cause infections such as pneumonia, skin infections, anthrax, and sinusitis. The objective of this study was to determine the phytochemical profile, antibacterial and antibiofilm efficacy of Swietenia macrophylla methanolic extract (SMME) against Gram-positive pathogens. The secondary metabolites of SMME were analyzed using GC-MS while the antibacterial efficacy of SMME against Staphylococcus aureus ATCC 33862, Bacillus cereus ATCC 11778, Streptococcus pneumonia ATCC 19615, and Clostridium sporogenes ATCC 13124 was assessed using MIC and MBC assays. Biofilm biomass assay and time-kill assay were performed to determine the antibiofilm activity of SMME against the pathogens. Results demonstrated that six common antibacterial secondary metabolites were present in the SMME. The major compound was found to be β-amyrin (22.8%). The SMME showed the lowest MIC values against B. cereus (31.25 µg/mL) and C. sporogenes (31.25 µg/mL) and the lowest MBC value against S. aureus (1000 µg/mL). The SMME also significantly (p<0.05) inhibited all the biofilms. It started to inhibit S. pneumonia and C. sporogenes biofilms after 12 h of exposure. On the other hand, the BIC50 value showed that the SMME was most effective against B. cereus. In conclusion, the secondary metabolites in the SMME may contribute to the antibacterial and antibiofilm efficacy against Gram-positive pathogens. © 2023 Malaysian Society of Applied Biology.
Malaysian Society of Applied Biology
1268643
English
Article
All Open Access; Bronze Open Access; Green Open Access
author Amran S.S.D.; Jalil M.T.M.; Aziz A.A.; Yahya M.F.Z.R.
spellingShingle Amran S.S.D.; Jalil M.T.M.; Aziz A.A.; Yahya M.F.Z.R.
Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
author_facet Amran S.S.D.; Jalil M.T.M.; Aziz A.A.; Yahya M.F.Z.R.
author_sort Amran S.S.D.; Jalil M.T.M.; Aziz A.A.; Yahya M.F.Z.R.
title Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
title_short Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
title_full Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
title_fullStr Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
title_full_unstemmed Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
title_sort Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens
publishDate 2023
container_title Malaysian Applied Biology
container_volume 52
container_issue 2
doi_str_mv 10.55230/mabjournal.v52i2.2642
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85164167532&doi=10.55230%2fmabjournal.v52i2.2642&partnerID=40&md5=df2b35266546e484108b8ca0d7414257
description Gram-positive pathogens cause infections such as pneumonia, skin infections, anthrax, and sinusitis. The objective of this study was to determine the phytochemical profile, antibacterial and antibiofilm efficacy of Swietenia macrophylla methanolic extract (SMME) against Gram-positive pathogens. The secondary metabolites of SMME were analyzed using GC-MS while the antibacterial efficacy of SMME against Staphylococcus aureus ATCC 33862, Bacillus cereus ATCC 11778, Streptococcus pneumonia ATCC 19615, and Clostridium sporogenes ATCC 13124 was assessed using MIC and MBC assays. Biofilm biomass assay and time-kill assay were performed to determine the antibiofilm activity of SMME against the pathogens. Results demonstrated that six common antibacterial secondary metabolites were present in the SMME. The major compound was found to be β-amyrin (22.8%). The SMME showed the lowest MIC values against B. cereus (31.25 µg/mL) and C. sporogenes (31.25 µg/mL) and the lowest MBC value against S. aureus (1000 µg/mL). The SMME also significantly (p<0.05) inhibited all the biofilms. It started to inhibit S. pneumonia and C. sporogenes biofilms after 12 h of exposure. On the other hand, the BIC50 value showed that the SMME was most effective against B. cereus. In conclusion, the secondary metabolites in the SMME may contribute to the antibacterial and antibiofilm efficacy against Gram-positive pathogens. © 2023 Malaysian Society of Applied Biology.
publisher Malaysian Society of Applied Biology
issn 1268643
language English
format Article
accesstype All Open Access; Bronze Open Access; Green Open Access
record_format scopus
collection Scopus
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