Kinetic Hydrolysis of Cellulose Biopolymer by Carbon Nanotubes Immobilized Cellulase

• Published in: Journal of Advanced Research in Applied Sciences and Engineering Technology
• Main Author: Azmi N.D.; Saat M.N.; Annuar M.S.M.
• Format: Article
• Language: English
• Published: Penerbit Akademia Baru 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163054216&doi=10.37934%2faraset.30.3.325333&partnerID=40&md5=d988a3489bfd272b6ea7b63914459eda

Immobilized enzymes are widely used in various biochemical reactions due to higher stability and reusability. Immobilization enzyme is a process of confinement enzyme molecules onto/within a support or matrix material via the chemical or physical process. In this study, the effect of free and CNT-Immobilized cellulase was investigated for hydrolysis of different CMC concentrations. The effect of free and CNT-Immobilized cellulase on the kinetic parameters Vmax and Km in the CMC hydrolysis was compared. The maximum reaction velocity Vmax of CNT-Immobilized cellulase is similar to free cellulase which indicates cellulase adsorption in CNT is effective in hydrolyzing CMC. However, the observed Km values for CNT-Immobilized cellulase is higher than free cellulase for different CMC concentrations. The Km value for CNT-Immobilized shows a sharp decrease in substrate affinity as compared to free cellulase due to diffusional restrictions by the CNT matrix. In addition, the initial velocity (V0) of CMC hydrolysis by CNT-Immobilized cellulase shows similar increasing pattern with the increase of reaction mixture viscosity. The current preparation of CNT-Immobilized cellulase have almost the same catalytic reaction in CMC hydrolysis as free cellulase. Thus, the current preparation of CNT-Immobilized has significant potential for green and sustainable biocatalyst in cellulose-based biopolymer hydrolysis reaction. © 2023, Penerbit Akademia Baru. All rights reserved.