Lateral Flow Immunoassays for SARS-CoV-2

The continued circulation of SARS-CoV-2 virus in different parts of the world opens up the possibility for more virulent variants to evolve even as the coronavirus disease 2019 transitions from pandemic to endemic. Highly transmissible and virulent variants may seed new disruptive epidemic waves tha...

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Bibliographic Details
Published in:Diagnostics
Main Author: Ang G.Y.; Chan K.G.; Yean C.Y.; Yu C.Y.
Format: Review
Language:English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2022
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149486365&doi=10.3390%2fdiagnostics12112854&partnerID=40&md5=6c82971a54a558dc42dfe31f5d7aced9
id 2-s2.0-85149486365
spelling 2-s2.0-85149486365
Ang G.Y.; Chan K.G.; Yean C.Y.; Yu C.Y.
Lateral Flow Immunoassays for SARS-CoV-2
2022
Diagnostics
12
11
10.3390/diagnostics12112854
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149486365&doi=10.3390%2fdiagnostics12112854&partnerID=40&md5=6c82971a54a558dc42dfe31f5d7aced9
The continued circulation of SARS-CoV-2 virus in different parts of the world opens up the possibility for more virulent variants to evolve even as the coronavirus disease 2019 transitions from pandemic to endemic. Highly transmissible and virulent variants may seed new disruptive epidemic waves that can easily put the healthcare system under tremendous pressure. Despite various nucleic acid-based diagnostic tests that are now commercially available, the wide applications of these tests are largely hampered by specialized equipment requirements that may not be readily available, accessible and affordable in less developed countries or in low resource settings. Hence, the availability of lateral flow immunoassays (LFIs), which can serve as a diagnostic tool by detecting SARS-CoV-2 antigen or as a serological tool by measuring host immune response, is highly appealing. LFI is rapid, low cost, equipment-free, scalable for mass production and ideal for point-of-care settings. In this review, we first summarize the principle and assay format of these LFIs with emphasis on those that were granted emergency use authorization by the US Food and Drug Administration followed by discussion on the specimen type, marker selection and assay performance. We conclude with an overview of challenges and future perspective of LFI applications. © 2022 by the authors.
Multidisciplinary Digital Publishing Institute (MDPI)
20754418
English
Review
All Open Access; Gold Open Access; Green Open Access
author Ang G.Y.; Chan K.G.; Yean C.Y.; Yu C.Y.
spellingShingle Ang G.Y.; Chan K.G.; Yean C.Y.; Yu C.Y.
Lateral Flow Immunoassays for SARS-CoV-2
author_facet Ang G.Y.; Chan K.G.; Yean C.Y.; Yu C.Y.
author_sort Ang G.Y.; Chan K.G.; Yean C.Y.; Yu C.Y.
title Lateral Flow Immunoassays for SARS-CoV-2
title_short Lateral Flow Immunoassays for SARS-CoV-2
title_full Lateral Flow Immunoassays for SARS-CoV-2
title_fullStr Lateral Flow Immunoassays for SARS-CoV-2
title_full_unstemmed Lateral Flow Immunoassays for SARS-CoV-2
title_sort Lateral Flow Immunoassays for SARS-CoV-2
publishDate 2022
container_title Diagnostics
container_volume 12
container_issue 11
doi_str_mv 10.3390/diagnostics12112854
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149486365&doi=10.3390%2fdiagnostics12112854&partnerID=40&md5=6c82971a54a558dc42dfe31f5d7aced9
description The continued circulation of SARS-CoV-2 virus in different parts of the world opens up the possibility for more virulent variants to evolve even as the coronavirus disease 2019 transitions from pandemic to endemic. Highly transmissible and virulent variants may seed new disruptive epidemic waves that can easily put the healthcare system under tremendous pressure. Despite various nucleic acid-based diagnostic tests that are now commercially available, the wide applications of these tests are largely hampered by specialized equipment requirements that may not be readily available, accessible and affordable in less developed countries or in low resource settings. Hence, the availability of lateral flow immunoassays (LFIs), which can serve as a diagnostic tool by detecting SARS-CoV-2 antigen or as a serological tool by measuring host immune response, is highly appealing. LFI is rapid, low cost, equipment-free, scalable for mass production and ideal for point-of-care settings. In this review, we first summarize the principle and assay format of these LFIs with emphasis on those that were granted emergency use authorization by the US Food and Drug Administration followed by discussion on the specimen type, marker selection and assay performance. We conclude with an overview of challenges and future perspective of LFI applications. © 2022 by the authors.
publisher Multidisciplinary Digital Publishing Institute (MDPI)
issn 20754418
language English
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accesstype All Open Access; Gold Open Access; Green Open Access
record_format scopus
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