Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray

One of the factors that contribute to the development of gastric cancer is the host immune response. Extensive immunophenotype of gastric cancer can be identified by using antibody microarray technique that profiles more than 100 cluster of differentiation (CD) antigens in parallel. In this study, w...

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Published in:Sains Malaysiana
Main Author: Hanafiah A.; Sukri A.; Kosai N.R.; Shuhaili M.A.; Taher M.M.; Ali R.A.R.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149015239&doi=10.17576%2fjsm-2023-5201-15&partnerID=40&md5=824d535227403e6e24f727e512208517
id 2-s2.0-85149015239
spelling 2-s2.0-85149015239
Hanafiah A.; Sukri A.; Kosai N.R.; Shuhaili M.A.; Taher M.M.; Ali R.A.R.
Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
2023
Sains Malaysiana
52
1
10.17576/jsm-2023-5201-15
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149015239&doi=10.17576%2fjsm-2023-5201-15&partnerID=40&md5=824d535227403e6e24f727e512208517
One of the factors that contribute to the development of gastric cancer is the host immune response. Extensive immunophenotype of gastric cancer can be identified by using antibody microarray technique that profiles more than 100 cluster of differentiation (CD) antigens in parallel. In this study, we used DotScanTM antibody microarray to profile CD antigen expression in patients with distinct digestive diseases for surface antigen disease-signatures. Patients' blood samples with gastric disorders and healthy controls were taken and processed for leukocytes isolation using Histopaque density gradient centrifugation. Leukocytes were captured onto DotScanTM slides and cell binding densities were analyzed using DotReaderTM. Different groups of gastric diseases were found to be characterized by differentially expressed distinct CD antigens. Compared to normal healthy controls, 17, two and four highly expressed CD antigens were identified in gastritis, gastric ulcer and gastric cancer patients, respectively. The 17 CD antigens that show differential expression in gastritis were CD15, CD16, CD20, CD23, CD24, CD25, CD28, CD34, CD37, CD77, CD102, CD103, CD122, CD128, CD10, CD183, and CD184. High expression of CD64 and CD69 were found in gastric ulcer, whereas CD52, CD126, CD135, and CD121a were highly expressed in gastric cancer. CD antigens involve in T-cell functions had reduced expression in gastric cancer, while proinflammatory cytokines shows increased expression. These results demonstrate specific immunophenotype of CD antigens in patients with various gastric diseases and identification of differential expressed surface antigens may have clinical significance for diagnostic and therapeutic purposes. © 2023 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.
Penerbit Universiti Kebangsaan Malaysia
1266039
English
Article
All Open Access; Gold Open Access
author Hanafiah A.; Sukri A.; Kosai N.R.; Shuhaili M.A.; Taher M.M.; Ali R.A.R.
spellingShingle Hanafiah A.; Sukri A.; Kosai N.R.; Shuhaili M.A.; Taher M.M.; Ali R.A.R.
Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
author_facet Hanafiah A.; Sukri A.; Kosai N.R.; Shuhaili M.A.; Taher M.M.; Ali R.A.R.
author_sort Hanafiah A.; Sukri A.; Kosai N.R.; Shuhaili M.A.; Taher M.M.; Ali R.A.R.
title Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
title_short Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
title_full Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
title_fullStr Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
title_full_unstemmed Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
title_sort Immunophenotyping of Gastritis, Gastric Ulcer and Gastric Cancer using a Cluster of Differentiation (CD) Antibody Microarray
publishDate 2023
container_title Sains Malaysiana
container_volume 52
container_issue 1
doi_str_mv 10.17576/jsm-2023-5201-15
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149015239&doi=10.17576%2fjsm-2023-5201-15&partnerID=40&md5=824d535227403e6e24f727e512208517
description One of the factors that contribute to the development of gastric cancer is the host immune response. Extensive immunophenotype of gastric cancer can be identified by using antibody microarray technique that profiles more than 100 cluster of differentiation (CD) antigens in parallel. In this study, we used DotScanTM antibody microarray to profile CD antigen expression in patients with distinct digestive diseases for surface antigen disease-signatures. Patients' blood samples with gastric disorders and healthy controls were taken and processed for leukocytes isolation using Histopaque density gradient centrifugation. Leukocytes were captured onto DotScanTM slides and cell binding densities were analyzed using DotReaderTM. Different groups of gastric diseases were found to be characterized by differentially expressed distinct CD antigens. Compared to normal healthy controls, 17, two and four highly expressed CD antigens were identified in gastritis, gastric ulcer and gastric cancer patients, respectively. The 17 CD antigens that show differential expression in gastritis were CD15, CD16, CD20, CD23, CD24, CD25, CD28, CD34, CD37, CD77, CD102, CD103, CD122, CD128, CD10, CD183, and CD184. High expression of CD64 and CD69 were found in gastric ulcer, whereas CD52, CD126, CD135, and CD121a were highly expressed in gastric cancer. CD antigens involve in T-cell functions had reduced expression in gastric cancer, while proinflammatory cytokines shows increased expression. These results demonstrate specific immunophenotype of CD antigens in patients with various gastric diseases and identification of differential expressed surface antigens may have clinical significance for diagnostic and therapeutic purposes. © 2023 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.
publisher Penerbit Universiti Kebangsaan Malaysia
issn 1266039
language English
format Article
accesstype All Open Access; Gold Open Access
record_format scopus
collection Scopus
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