A voltammetric immunosensor based on a nanoporous alumina millirod for detection of porcine serum albumin

• Published in: Food Chemistry
• Main Author: Mahmud A.H.; Salahuddin N.M.; Md Jani A.M.; Abu Bakar N.F.; Zainal Abidin S.A.S.; Mohd Zain Z.; Low K.-F.
• Format: Article
• Language: English
• Published: Elsevier Ltd 2023
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85147457841&doi=10.1016%2fj.foodchem.2023.135493&partnerID=40&md5=a4d5f228edc48b0121660ffaef9bdc43

A voltammetric immunosensor was developed for detection of porcine serum albumin (PSA) to identify raw meat products adulterated with pork. A novel strategy to fabricate multiple individual nanoporous alumina (NPA) millirods (length, 5.0 mm; diameter, 1.0 mm) as the biorecognition platform is described. Each NPA millirod was covalently bioconjugated with anti-PSA capturing antibodies (α-PSAC). Following immunocapture, the PSA bound to the α-PSAC/NPA millirod bioconjugate were tagged with gold nanoparticles (AuNPs) functionalized with anti-PSA detection antibodies as the signaling probe. Subsequently, the AuNPs were voltammetrically analyzed to quantify the target PSA. The immunosensor exhibited 100 % specificity and high sensitivity to PSA with a limit of detection (LoD) of 50 (range, 0–1000) pg/mL (R2 = 0.9907). Real-world applicability was successfully validated using pork/beef adulterated mixtures with a LoD of 0.05 % (w/w). Overall, the detection performance of the proposed immunosensor was excellent and, thus, is suitable for surveillance of food safety and quality. © 2023 Elsevier Ltd